具有近红外光热转换性能的双层氧化硅包覆金纳米花复合材料的制备及体内代谢研究

doi:10.7503/cjcu20180299

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (12): 2644.doi: 10.7503/cjcu20180299

具有近红外光热转换性能的双层氧化硅包覆金纳米花复合材料的制备及体内代谢研究

宋文植², 李慧², 张艳², 何丹¹, 李英姿², 黄臻臻³, 刘新², 尹万忠¹()

1. 吉林大学第一临床医院, 长春 130021
2. 吉林大学中日联谊医院, 长春 130031
3. 吉林大学化学学院, 长春 130012

收稿日期:2018-04-16 出版日期:2018-12-03 发布日期:2018-06-26
作者简介:
联系人简介: 尹万忠, 男, 博士, 副教授, 主要从事纳米材料在头颈肿瘤诊疗中的应用研究. E-mail: yinwz@jlu.edu.cn
基金资助:
国家自然科学基金(批准号: 81372900)、吉林省科技发展计划项目(批准号: 20110708,20180414014GH)和2014年吉林大学交叉创新择优资助项目资助.

Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo^†

SONG Wenzhi², LI Hui², ZHANG Yan², HE Dan¹, LI Yingzi², HUANG Zhenzhen³, LIU Xin², YIN Wanzhong^1,^*()

1. The First Clinical Hospital of Jilin University, Changchun 130021, China
2. China-Japan Union Hospital of Jilin University, Changchun 130031, China
3. College of Chemistry, Jilin University, Changchun 130012, China

Received:2018-04-16 Online:2018-12-03 Published:2018-06-26
Contact: YIN Wanzhong E-mail:yinwz@jlu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.81372900), the Science and Technology Development Plan of Jilin Province, China(Nos.20110708, 20180414014GH) and the Preferential Cross Innovation Project of Jilin University, China(2014).

摘要/Abstract

摘要：

采用种子生长法和改良Stöber法制备了双层氧化硅包覆的金纳米花复合材料, 并测试了其近红外光热转换性能, 研究了其在裸鼠体内的生物毒性, 并以种植了人口腔鳞癌CAL27细胞的荷瘤裸鼠为体内模型, 应用光声成像方法研究了其体内分布、代谢和在肿瘤组织的被动靶向富集行为. 结果表明, 所制备的金纳米花复合材料在近红外光区具有良好的光热转换性能, 无明显体内生物毒性, 是一种良好的光声成像剂, 其在荷瘤裸鼠体内主要经肝脏代谢至肠道排出体外, 可通过EPR效应实现在肿瘤组织的靶向富集. 本研究证明所制备的双层氧化硅包覆金纳米花复合材料可用于生物体内治疗, 可作为包括口腔癌在内的恶性肿瘤近红外光热诊疗的理想介导材料.

关键词: 金纳米花-二氧化硅复合粒子, 核壳结构纳米粒子, 光声成像, 近红外光热转换, 口腔癌

Abstract:

Gold nanoflower-double layer silica core-shell nanoparticles were prepared by a seed-mediated growth strategy and a modified Stöber method. The photothermal properties and the biotoxicity in vivo of the as-prepared core-shell nanoparticles were studied. The nude mice bearing oral cancer cell CAL27 were taken as the animal model to investigate the distribution, metabolism and passive targeting enrichment of the core-shell nanoparticles in vivo by the photoacoustic imaging technique. The results showed that the as-prepared core-shell nanoparticles exhibited excellent photothermal conversion properties in near infrared(NIR) region. The core-shell nanoparticles could be well metabolized in the liver, and could be excreted outside of the body through the intestinal tract. More importantly, the core-shell nanoparticles exhibited passive targeting enrichment in the tumor tissue through EPR effect. Our study show that the gold nanoflower-double layer silica core-shell nanoparticles would be an ideal photoacoustic imaging agent and a NIR photothermal therapy agent for diagnose and therapy of malignant tumors such as oral cancer.

Key words: Gold nanoflower-silica nanocomposites, Core-shell nanoperticles, Photoacustic imaging(PAI), Near infrared(NIR) photothermal conversion, Oral cancer

中图分类号:

O614
O644

TrendMD:

宋文植, 李慧, 张艳, 何丹, 李英姿, 黄臻臻, 刘新, 尹万忠. 具有近红外光热转换性能的双层氧化硅包覆金纳米花复合材料的制备及体内代谢研究. 高等学校化学学报, 2018, 39(12): 2644.

SONG Wenzhi,LI Hui,ZHANG Yan,HE Dan,LI Yingzi,HUANG Zhenzhen,LIU Xin,YIN Wanzhong. Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo^†. Chem. J. Chinese Universities, 2018, 39(12): 2644.

图/表 6

Scheme 1 Synthesis of AuNF@SiO2@mSiO2 nanoparticles

Fig.1 TEM image(A) and UV-Vis spectrum(B) of the AuNF@SiO2@mSiO2 nanoparticles

Fig.2 Photo-thermal conversion curves of the aqueous dispersions of AuNF@SiO2@mSiO2 nanoparticles with different concentrations under the NIR laser irradiation with power of 5 W/cm2(A), 7 W/cm2(B) and 9 W/cm2(C)a. Control; b. 50 μg/mL; c. 100 μg/mL; d. 150 μg/mL; e. 200 μg/mL; f. 250 μg/mL.

Fig.3 Morphological observation on different organs of the nude mice(A1—D1): control group; (A2—D2): test group. (A1, A2): liver; (B1, B2): heart; (C1, C2): kidney; (D1, D2): spleen.

Table 1 Serum biochemical indices of the nude mice injected with AuNF@SiO2@mSiO2 nanoparticles

Group	c(BUN)/ (μmol·L^-1)	c(Cr)/ (μmol·L^-1)	c(GPT)/ (U·L^-1)	c(GOT)/ (U·L^-1)	c(CK)/ (U·mL^-1)	c(CK-MB)/ (U·L^-1)	c(LDH)/ (U·L^-1)
Experimental	2.121±0.066	55.975±1.234	110.245±3.075	205.989±6.022	0.911±0.058	2010.113±78.94	893.717±20.256
Control	2.158±0.091	54.70±1.264	108.843±2.587	201.655±4.578	0.877±0.041	1962.32±63.825	882.432±23.278

Fig.4 Photoacoustic imaging of the living CAL27-tumour-bearing mice after tail intravenous injection of AuNF@SiO2@mSiO2 nanoparticles

参考文献 37

[1]	Torre L.A., Bray F., Siegel R.L., Ferlay J., Lortet-Tieulent J., Jemal A., CA Cancer J.Clin., 2015, 65(2), 87—108
[2]	Jain P.K., Lee K.S., El-Sayed I.H., El-Sayed M. A.J., Phys. Chem.B,2006, 110(14), 7238—7248
[3]	Saverot S.E., Reese L.M., Cimini D., Vikesland P.J., Bickford L.R., Nanoscale Res. Lett.,2015, 10, 241—254
[4]	Fay B.L., Melamed J.R., Day E.S., Int. J. Nanomedicine,2015, 10, 6931—6941
[5]	Song W.Z., Jiang Y.P., Ji X.H., Zhao L.L., Yin W.Z., Yang W.S., Chem. J. Chinese Universities,2012, 33(9), 1886—1888
	(宋文植, 姜雅萍, 纪小会, 赵丽丽, 尹万忠, 杨文胜. 高等学校化学学报, 2012, 33(9), 1886—1888)
[6]	Huang H., Yang D.P., Liu M.H., Wang X.S., Zhang Z.Y., Zhou G.D., Liu W., Cao Y.L., Zhang W.J., Wang X.S., Int. J. Nanomedicine,2017, 12, 2829—2843
[7]	Gobin A.M., Moon J.J., West J.L., Int. J. Nanomedicine,2008, 3(3), 351—358
[8]	Manivasagan P., Bharathiraja S., Moorthy M.S., Oh Y.O., Song K., Seo H., Oh J., ACS Appl. Mater. Interfaces,2017, 9(17), 14633—14646
[9]	Rejiya C.S., Jatish K., Raji V., Vibin M., Annie A., Pharmacol. Res.,2012, 65(2), 261—269
[10]	Huang X., El-Sayed I.H., Qian W., El-Sayed M. A., J. Am. Chem. Soc., 2006, 128, 2115—2120
[11]	Jiao P., Otto M., Geng Q., Li C., Li F., Butch E.R., Snyder S.E., Zhou H., Yan B., J.Mater.Chem., 2016, 4(3), 513—520
[12]	Lin A.Y., Young J.K., Nixon A.V., Drezek R.A., Small,2014, 10(16), 3246—3251
[13]	Luo T., Huang P., Gao G., Shen G., Fu S., Cui D., Zhou C., Ren Q., Opt. Express,2011, 19(18), 17030—17039
[14]	Yi S.J., Sun L.M., Lenaghan S.C., Wang Y.Z., Chong X.Y., Zhang Z.L., Zhang M.J., RSC Adv.,2013, 3(26), 10139—10144
[15]	Chen J., Sheng Z., Li P., Wu M., Zhang N., Yu X.F., Wang Y., Hu D., Zheng H., Wang G.P., Nanoscale,2017, 9(33), 11888—11901
[16]	Zhou G., Xiao H., Li X., Huang Y., Song W., Song L., Chen M., Cheng D., Shuai X., Acta Biomater.,2017, 64, 223—236
[17]	Sun Y., Wang Q., Chen J., Liu L., Ding L., Shen M Li J., Han B., Duan Y., Theranostics,2017, 7(18), 4424—4444
[18]	Sun Q., You Q., Pang X., Tan X., Wang J., Liu L., Guo F., Tan F., Li N., Biomaterials,2017, 122, 188—200
[19]	Cheng B., He H., Huang T., Berr S.S., He J., Fan D., Zhang J., Xu P., J. Biomed. Nanotechno.,2016, 12(3), 435—449
[20]	Huang Y., Rosei F., Vetrone F., Nanoscale,2015, 7(12), 5178—5185
[21]	Stassi S., Cauda V., Canavese G., Manfredi D., Pirri C.F., Eur. J. Inorg. Chem.,2012, 16, 2669—2673
[22]	Haiss W., Thanh N. T.K., Aveyard J., Fernig D.G., Anal. Chem.,2007, 79(11), 4215—4221
[23]	Stöber W., Fink A., Bohn E., J. Colloid Interface Sci.,1968, 26(1), 62—69
[24]	Yang J.P., Zhang F., Chen Y.R., Qian S., Hu P., Li W., Deng Y.H., Fang Y., Han L., Mohammad L., Zhao D.Y., Chem. Commun.,2011, 47(42), 11618—11620
[25]	Lai C.Y., Trewyn B.G., Jeftinija D.M., Jeftinija K., Xu S., Jeftinija S., Lin V.S., J. Am. Chem. Soc.,2003, 125(15), 4451—4459
[26]	Feng J., Wang Z., Shen B., Zhang L., Yang X., He N., RSC Adv.,2014, 4(54), 28683—28690
[27]	Austin L.A., Mackey M.A., Dreaden E.C., Elsayed M.A., Arch. Toxicol.,2014, 88(7), 1391—1417
[28]	Li W., Chen X., Nanomedicine,2015, 10(2), 299—320
[29]	Xu M., Wang L.V., Rev. Sci. Instrum.,2006, 77, 041101
[30]	Andreev V.G., Karabutov A.A., OraevskyA. A., IEEE Trans. Ultrason. Ferroelectr. Freq. Control,2003, 50(10), 1383—1390
[31]	Wang L.V., Nat. Photonics, 2009, 3(9), 503—509
[32]	Erpelding T.N., Kim C., Pramanik M., Jankovic L., Maslov K., Guo Z., Margenthaler J.A., Pashley M.D., Wang L.V., Radiology,2010, 256(1), 102—110
[33]	Kim C., Cho E.C., Chen J., Song K.H., Au L., Favazza C., Zhang Q., Cobley C.M., Gao F., Xia Y., Wang L.V., ACS Nano,2010, 4(8), 4559—4564
[34]	Emelianov S.Y., Li P.C., O’Donnell M., Phys. Today,2009, 62(8), 34—39
[35]	Zhang E.Z., Povazay B., Laufer J., Alex A., Hofer B., Pedley B., Glittenberg C., Treeby B., Cox B., Beard P., Drexler W., Biomed. Opt. Express,2011, 2(8), 2202—2215
[36]	Jansen K., van der Steen A. F. W., van Beusekom H. M. M., Oosterhuis J.W., van Soest G., Opt. Lett.,2011, 36(5), 597—599
[37]	Maeda H., Nakamura H., Fang J., Adv. Drug Deliv. Rev.,2013, 65(1), 71—79

具有近红外光热转换性能的双层氧化硅包覆金纳米花复合材料的制备及体内代谢研究

Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo^†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 37

相关文章 1

编辑推荐

Metrics

本文评价

具有近红外光热转换性能的双层氧化硅包覆金纳米花复合材料的制备及体内代谢研究

Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 37

相关文章 1

编辑推荐

Metrics

本文评价

Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo^†