高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (5): 873.doi: 10.7503/cjcu20150922

• 分析化学 • 上一篇    下一篇

基于液体阵列比色传感器鉴别不同茶叶的新方法

霍丹群1(), 付贝贝1, 李俊杰1, 杨眉1, 沈才洪2, 罗惠波3, 侯长军1()   

  1. 1. 生物流变科学与技术教育部重点实验室, 重庆大学生物工程学院, 重庆 400044
    2. 国家固态酿造工程技术研究中心, 泸州646000
    3. 四川理工学院生物工程学院, 自贡 643000
  • 收稿日期:2015-11-30 出版日期:2016-05-10 发布日期:2016-04-07
  • 作者简介:联系人简介: 霍丹群, 女, 博士, 教授, 博士生导师, 主要从事卟啉生化传感器研究. E-mail:huodq@cqu.edu.cn
    侯长军, 男, 博士, 教授, 博士生导师, 主要从事生化传感与系统研究. E-mail: houdcj@cqu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 31171684)、 重庆市研究生科研创新项目(批准号: CYS15010)、 酿酒生物技术及应用四川省重点实验室项目(批准号: NJ2014-03)和重庆大学大型仪器设备开放基金资助

New Method for Identifying Different Teas Based on Liquid Colorimetric Sensor

HUO Danqun1,*(), FU Beibei1, LI Junjie1, YANG Mei1, SHEN Caihong2, LUO Huibo3, HOU Changjun1,*()   

  1. 1. Key Laboratory of Biorheology Science and Technology, Ministry of Education, College of Bioengineering,Chongqing University, Chongqing 400044, China
    2.National Engineering Research Center of Solid-State Brewing, Luzhou 646000, China
    3. College of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, China
  • Received:2015-11-30 Online:2016-05-10 Published:2016-04-07
  • Contact: HUO Danqun,HOU Changjun E-mail:huodq@cqu.edu.cn;houdcj@cqu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.31171684), the Chongqing Graduate Student Research Innovation Project of China(No.CYS15010), the Liquor Making Biology Technology and Application of Key Laboratory Program of Sichuan Province of China(No.NJ2014-03) and the Sharing Fund of Chongqing University’s Large Equipment of China

摘要:

基于茶多酚与三价铁的络合性以及对氧化铈的还原性, 构建了交叉响应的液体阵列传感器, 为茶叶种类的区分提供了一种新方法. 利用水热法合成了硅酸锌空心微球和纳米棒作为三价铁的吸附剂, 同时合成了可被茶多酚还原的纳米氧化铈, 以硅酸锌-硫氰酸铁和氧化铈共同构建3×3阵列. 对5种茶多酚和14种茶叶进行了检测, 每个样本做5次平行实验, 通过聚类分析(HCA)和主成份分析(PCA)方法对反应前后的图谱进行了分析. 结果表明, 该阵列能够将结构类似的茶多酚化合物准确区分, 并且平行样本之间稳定性较好; 同时对不同品种的茶叶样本区分效果良好, 结合反应前后的差谱图可以实现对茶叶的定性分析. 该方法在茶叶品质和食品质量的快速可视化检测方面具有潜在应用价值.

关键词: 茶多酚, 比色传感, 硅酸锌纳米材料, 纳米氧化铈, 硫氰酸铁

Abstract:

A liquid colorimetric sensor array was presented for cross-reactive discrimination of tea fusions based on complexation reaction and redox reaction of tea polyphenols with ferric ions and ceria nanoparticles, respectively. Hydrothermal reaction was applied to synthesize different kinds of ceria nanoparticles, hollow nanospheres and nanorods of zinc silicate to immobilized ferric thiocyanate[Fe(SCN)3], which were subsequently combined to construct a 3×3 colorimetric sensor array. Performance of the colorimetric sensor array was evaluated using 5 kinds of tea polyphenols and 14 kinds of tea fusions with 5 repeated parallels. Hierarchical clustering analysis(HCA) and principal component analysis(PCA) of the colorimetric profiles suggested that all 5 kinds of tea polyphenols can be easily differentiated with good reproducibility. Correct discrimination of 14 kinds of tea fusions was also realized according to individual quality grade and categories. Our preliminary study provides a potential alternative for fast visible detection and discrimination of teas within different qualities and grades.

Key words: Tea polyphenol, Colorimetric sensor, Zinc silicate nanomaterial, Ceria nanoparticles, Ferric thiocyanate

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