高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (7): 1541-1545.doi: 10.7503/cjcu20131252

• 物理化学 • 上一篇    下一篇

Ag/mSiO2空心微球的制备及SERS标记性能

任志宇(), 李忠涛, 杜世超, 吴君, 孔令俊, 付宏刚   

  1. 黑龙江大学功能无机材料化学教育部重点实验室, 化学化工与材料学院, 哈尔滨 150080
  • 收稿日期:2013-12-19 出版日期:2014-07-10 发布日期:2014-04-29
  • 作者简介:联系人简介: 任志宇, 女, 博士, 副教授, 主要从事纳米复合材料合成及生物检测研究. E-mail: zyren@hlju.edu.cn
  • 基金资助:
    黑龙江省普通高等学校青年学术骨干支持计划项目(批准号: 1251G045)资助

Synthesis of Ag/mSiO2 Hollow Microspheres for SERS-tags

REN Zhiyu*(), LI Zhongtao, DU Shichao, WU Jun, KONG Lingjun, FU Honggang   

  1. Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, China
  • Received:2013-12-19 Online:2014-07-10 Published:2014-04-29
  • Contact: REN Zhiyu E-mail:zyren@hlju.edu.cn

摘要:

以聚苯乙烯微球为模板, 经过原位还原和种子生长过程在聚苯乙烯微球表面包覆银(Ag)纳米粒子; 以正硅酸乙酯为硅源, 在十六烷基三甲基溴化铵的导向下实现介孔二氧化硅(mSiO2)可控包覆, 去除模板得到Ag/mSiO2空心微球. 透射电子显微镜(TEM)和氮气吸附-脱附分析结果表明, SiO2壳层厚度约为20 nm, 介孔孔径为2.1 nm, 孔道分布均匀. 进一步利用虹吸作用使对巯基苯胺(4-ATP)分子进入微球内与Ag粒子结合, 构建表面增强拉曼散射(SERS)标记材料. SERS测试结果表明, 该标记材料检测限达到10-7 mol/L, SERS增强因子达到3.7×105.

关键词: 银, 介孔二氧化硅, 空心微球, 表面增强拉曼散射标记

Abstract:

Ag/mSiO2 hollow microspheres were synthesized via the following steps. Monodisperse polystyrene(PS) colloidal microspheres were coated with Ag nanoparticles through in situ reduction and growing seeds. The composite microspheres were then controllably coated with mesoporous silica shell using cetyl trimethyl ammonium bromide(CTAB) as pore-forming agent. After getting rid of template, Ag/mSiO2 hollow microspheres were obtained. To actualize SERS-tags detection, the hollow microspheres were subsequently tagged with 4-ATP via siphonage effect. Due to the air-resistance effect of mesoporous channel and light’s multiple scattering in the interior of hollow microspheres, the obtained SERS-tags material showed low limit of detection and high stability.

Key words: Ag, Mesoporous silica, Hollow microspheres, SERS-tags

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