高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (6): 1374-1378.doi: 10.7503/cjcu20120824

• 分析化学 • 上一篇    下一篇

复方乌头汤配伍贝母化学成分变化研究

齐瑶1,2, 李世哲3, 朱洪彬1,2, 宋凤瑞1, 林娜4, 刘志强1   

  1. 1. 中国科学院长春应用化学研究所, 长春 130022;
    2. 中国科学院大学, 北京 100039;
    3. 长春师范大学化学学院, 长春 130022;
    4. 中国中医科学院中药研究所, 北京 100700
  • 收稿日期:2012-09-10 出版日期:2013-06-10 发布日期:2013-05-07
  • 通讯作者: 刘志强,男,博士,研究员,博士生导师,主要从事天然药物化学与有机质谱学研究.E-mail:liuzq@ciac.jl.cn E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 81073040, 81274046)和国家"九七三"计划项目(批准号: 2011CB505300, 2011CB505305)资助.

Chemical Constituent Changes of Wu-tou-tang in Compatibility Process with Fritillariae cirrhosae and Fritillariae thunbergii

QI Yao1,2, LI Shi-Zhe3, ZHU Hong-Bin1,2, SONG Feng-Rui1, LIN Na4, LIU Zhi-Qiang1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. University of the Chinese Academy of Sciences, Beijing 100039, China;
    3. College of Chemistry, Changchun Normal University, Changchun 130022, China;
    4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2012-09-10 Online:2013-06-10 Published:2013-05-07

摘要:

将超高效液相色谱-质谱联用(UPLC-MS) 及UPLC-MSn技术与偏最小二乘法判别分析(PLS-DA)结合, 对乌头汤分别与川贝、 浙贝按照制川乌与贝母生药质量比1: 1配伍前后化学成分的变化情况进行了研究. 在PLS-DA模型中, 乌头汤与川贝、 浙贝配伍前后的煎煮液均可明显区分. 乌头汤与川贝共煎后获得8个化学标志物, 可鉴定出7个化合物, 其中尼奥灵、 附子灵及苯甲酰基新乌头原碱在共煎后含量上升, 塔拉定、 10-OH苯甲酰基乌头原碱、 10-OH苯甲酰基新乌头原碱和苯甲酰基去氧乌头原碱在配伍后含量下降. 乌头汤与浙贝共煎后获得7个化学标志物, 分别为Chuanfumine、 Songorine、 塔拉定、 尼奥灵、 附子灵、 苯甲酰基新乌头原碱和10-OH苯甲酰基新乌头原碱, 所有化学标志物的含量在共煎后均降低.

关键词: 乌头汤, 川贝, 浙贝, 十八反, 超高效液相色谱-质谱联用

Abstract:

Wu-tou-tang was chosen to investigate the chemical constituent changes of Aconitum-Fritillariae combination taboo in prescription. The ultra performance liquid chromatography coupled with mass spectrometry(UPLC-MS) and tandem mass spectrometry methods were used to evaluate the chemical composition of Wu-tou-tang and its co-decoction with Fritillariae cirrhosae and Fritillariae thunbergiiin with a mass ratio of 1: 1. The total ion chromatograms(TIC) of the co-decoctions were processed by Mzmine 2, and then the dataset was analyzed by partial least squares discriminant analysis(PLS-DA). Significant differences between the group of Wu-tou-tang and Wu-tou-tang combined with Fritillariae cirrhosae and Fritillariae thunbergii in PLS-DA model were observed. Seven of the eight potential chemical markers in Wu-tou-tang and Fritillariae cirrhosae compa-tibility process were identified, the relative content of neoline, fuziline and benzoylmesaconine increased and talatizidine, 10-OH benzoylmesaconine, 10-OH benzoylaconine and benzoyldeoxyaconine decreased. Chuanfumine, songorine, talatizidine, neoline, fuziline, benzoylmesaconine and 10-OH benzoylmesaconine were found as potential chemical markers in Wu-tou-tang and Fritillariae thunbergii compatibility process, respectively. The result shows that relative content of all potential chemical markers in Wu-tou-tang and Fritillariae thunbergii co-decoction was less than that in Wu-tou-tang.

Key words: Wu-tou-tang, Fritillariae cirrhosae, Fritillariae thunbergii, Eighteen incompatible medicaments, Ultra performance liquid chromatography coupled with mass spectrometry(UPLC-MS)

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