高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (10): 2169.doi: 10.7503/cjcu20120249

• 分析化学 • 上一篇    下一篇

格列美脲治疗的2型糖尿病大鼠的尿液代谢组学研究

牛俊1,2, 皮子凤1, 越皓3, 刘淑莹1,3   

  1. 1. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
    2. 中国科学院研究生院, 北京 100049;
    3. 长春中医药大学, 长春 130117
  • 收稿日期:2012-03-21 出版日期:2012-10-10 发布日期:2012-09-12
  • 通讯作者: 刘淑莹, 女, 博士, 研究员, 博士生导师, 主要从事生物质谱研究. E-mail: syliu19@yahoo.com.cn E-mail:syliu19@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号: 21005075, 21175127); 吉林省科技发展计划(批准号: 20090739, 20101501)和吉林省医药产业发展专项资金(批准号: YYZX201131)资助.

Urinary Metabonomics Study of Type 2 Diabetic Rats Treated by Glimepiride

NIU Jun1,2, PI Zi-Feng1, YUE Hao3, LIU Shu-Ying1,3   

  1. 1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China;
    3. Changchun University of Chinese Medicine, Changchun 130117, China
  • Received:2012-03-21 Online:2012-10-10 Published:2012-09-12

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被引次数

34

摘要：

采用快速高分离度液相色谱-质谱技术(RRLC-MS)检测经格列美脲治疗的2型糖尿病大鼠尿液中代谢物的变化, 对糖尿病组、 给药组和健康对照组大鼠的尿液代谢物谱进行了分析. 采用主成分分析(PCA)对3组大鼠进行分类并寻找潜在生物标记物. 结果表明, 3组大鼠的尿液代谢物谱得到了很好的区分, 发现并鉴定了2个潜在生物标记物, 分别为4-脱氧三羟基丁酸和4-胍基丁酸. 格列美脲对2型糖尿病大鼠的药物作用可能体现为对氨基酸代谢的调节作用.

关键词: 2型糖尿病大鼠, 代谢组学, 快速高分离度液相色谱-质谱, 格列美脲

Abstract:

A urinary metabonomics method based on rapid resolusion liquid chromatography-mass spectrometry(RRLC-MS) was developed to study the effect mechanism of glimepiride on type 2 diabetes mellitus. With multivariate statistical analysis of urinary metabolite profiles, a clear separation between streptozotocin-induced type 2 diabetic rats and those treated with glimepiride was achieved. Two significantly changed metabolites have been identified and used to explain the mechanism. The results show that the possible effect mechanism of glimepiride on type 2 diabetes is involved in amino acid metabolism.

Key words: Type 2 diabetic rat, Metabonomics, Rapid resolution liquid chromatography-mass spectrometry(RRLC-MS), Glimepiride

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