高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (07): 1420-1425.doi: 10.3969/j.issn.0251-0790.2012.07.010

• 分析化学 • 上一篇    下一篇

基于双纳米金探针杂交法检测HBV DNA

邢亚斯1,2, 邹能利1, 毛红菊1, 徐霞2, 葛玉卿1, 金庆辉1, 赵建龙1   

  1. 1. 中国科学院上海微系统与信息技术研究所传感技术联合国家重点实验室, 上海 200050;
    2. 郑州大学药学院, 郑州 450001
  • 收稿日期:2011-08-22 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 毛红菊, 女, 博士, 研究员, 主要从事生物传感器及纳米技术在重大疾病诊断治疗方面研究. E-mail: hjmao@mail.sim.ac.cn E-mail:hongjumao2002@yahoo.com.cn
  • 基金资助:

    上海市科学技术委员会纳米专项(批准号: 11nm0505800, 1052nm061000) 和国家自然科学基金(批准号: 31000791)资助.

Detection of Hepatitis B Virus DNA Hybridization Based on Dual Gold Nano-probes

XING Ya-Si1,2, ZOU Neng-Li1, MAO Hong-Ju1, XU Xia2, GE Yu-Qing1, JIN Qing-Hui1, ZHAO Jian-Long1   

  1. 1. State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, China;
    2. College of Pharmacy, Zhengzhou University, Zhengzhou 450001, China
  • Received:2011-08-22 Online:2012-07-10 Published:2012-07-10

摘要: 利用双纳米金探针结合基因芯片平台建立了一种检测乙肝病毒基因(HBV DNA)的新方法. 根据HBV DNA的保守序列设计捕获探针和信号报告探针, 通过一对互补的纳米金检测探针的双杂交法对HBV DNA进行信号放大, 最后进行银染, 达到对HBV DNA的可视化检测. 该方法的灵敏度高, 可检测10 fmol/L的HBV DNA, 且能在1.5 h内完成检测. 其具有的快速、 高灵敏度及低成本等优势使其有望发展成为一种检测HBV DNA的新方法.

关键词: 双纳米金探针, 杂交, 基因芯片, 银染, 乙肝病毒基因(HBV DNA)

Abstract: A novel Hepatitis B virus(HBV) DNA detection method based on dual gold nano-probes and gene chip was developed. Two types of probes including capture probe and reporter probe were designed according to conservative HBV DNA. A pair of complementary gold nanoparticles probes was used to amplify the signal of HBV DNA and visualized by silver staining on gene chip. The optimized detection limit of this method was 10 fmol/L, and the entire test could be completed in 1.5 h. This strategy could be potentially used for HBV DNA detection due to its rapid, high-sensitivity and low-cost properties.

Key words: Dual gold nano-probes, Hybridization, Gene chip, Silver staining, Hepatitis B virus(HBV) DNA

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