高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (02): 251.doi: 10.3969/j.issn.0251-0790.2012.02.007

• 分析化学 • 上一篇    下一篇

固定光路可变焦宽调角表面等离子共振成像装置

张轶鸣1, 陈畯1, 廖滔1, 许吉英1, 陈义1,2   

  1. 1. 中国科学院化学研究所活体分析化学重点实验室;
    2. 北京分子科学国家实验室, 北京100190
  • 收稿日期:2011-03-22 出版日期:2012-02-10 发布日期:2012-01-13
  • 通讯作者: 陈 义, 男, 博士, 研究员, 博士生导师, 主要从事高效高通量生物微分析研究. E-mail: chenyi@iccas.ac.cn E-mail:chenyi@iccas.ac.cn
  • 基金资助:

    国家自然科学基金(批准号: 21027003, 20805051)、国家"九七三"计划项目(批准号: 2007CB714504)和中国科学院知识创新工程重要方向项目(批准号: KJCX2-EW-N06-01)资助.

Surface Plasmon Resonance Imaging System with Fixed and Compact Optical Path, 2× Zoom Mechanism and Wide Variable Incident Angle

ZHANG Yi-Ming1, CHEN Jun1, LIAO Tao1, XU Ji-Ying1, CHEN Yi1,2   

  1. 1. Key Lab of Analytical Chemistry for Living Biosystem, Institue of Chemistry, Chinese Academy of Sciences;
    2. Beijing National Laboratory of Molecular Science, Beijing 100190, China
  • Received:2011-03-22 Online:2012-02-10 Published:2012-01-13

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被引次数

6

摘要： 设计了一种折反式棱镜耦合单元, 用于简化表面等离子共振系统的角度调节操作, 并基于此单元构建了固定光路可变焦宽调角表面等离子共振成像装置. 该装置通过旋转折反式棱镜耦合单元即可实现角度调节, 调节范围可达40°~80°, 结合1~2倍的变焦成像系统, 可对1~4 cm2传感芯片成像, 且可分辨至少3600 Point/cm2. 以蔗糖溶液为研究对象, 考察了该装置的灵敏度, 结果表明其灵敏度可达7×10-6 RIU(Refractive index unit), 共振角与折射率的线性相关系数为0.99953. 将该装置用于研究抗牛血清白蛋白抗体与其它蛋白的实时相互作用, 所得结果与理论相符. 该表面等离子共振成像装置将在实时并行分析方面具有广阔的应用前景.

关键词: 表面等离子共振成像装置, 固定光路, 广角, 折反式棱镜耦合

Abstract: A new surface plasmon resonance imaging(SPRi) system was designed and fabricated using a right angle trapezoid prism as SPR coupler, which allows the fixation of left optical elements in a compact space and addition of a 1—2× zooming adjuster. This design simplifies the angle scanning mechanism, enables a very fine adjustment of incident angle from 40° to 80° at 0.001° angle resolution, and makes the observation possible of size-variable chips(1—4 cm2), providing a lateral spatial resolution up to 3600 Point/cm2. Its sensiti-vity was evaluated with sucrose solutions, giving the data up to 7×10-6 RIU(Refractive index unit). A linear relationship was obtained between resonance angle and sample refractive index, with correlation coefficients of 0.99953. The new system has been further validated in the immuno-reaction of BSA with anti-BSA, which enables to produce both of kinetic reaction curves and differentiated dot images. These results indicate that the newly designed SPRi system is highly promising for high-through binding affinity applications.

Key words: Surface plasmon resonance imaging setup, Fixed-optical, Wide variable incident angle, Rightangle trapezoid prism coupler

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