Preparation of Bovine Hemoglobin-imprinted Polymer Beads via Photografting Surface-Modified Method

Chem. J. Chinese Universities

• 研究论文 • Previous Articles Next Articles

Preparation of Bovine Hemoglobin-imprinted Polymer Beads via Photografting Surface-Modified Method

GAI Qing-Qing^1,3, LIU Qiu-Ye¹, LI Wen-You¹, HE Xi-Wen¹*, CHEN Lang-Xing¹, ZHANG Yu-Kui^1,2*

1. Department of Chemistry, Nankai University, Tianjin 300071, China;
2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China;
3. Department of Pharmacology, Changzhi Medical College, Changzhi 046000, China

Received:2007-04-28 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
Contact: HE Xi-Wen

Abstract

Abstract: Molecularly imprinted polymers(MIP) based on photografting surface-modified polystyrene beads as matrices were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and N,N′-methylene bisacrylamide as the crosslinker in a phosphate buffer. The results of IR, scanning electron microscope(SEM) and elemental analysis demonstrate the formation of a grafting polymer layer on the polystyrene-bead surface. Subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. And the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. Attempt to employ the new method in molecular imprinting techniques may indroduce new application to MIPs and facilicate the probable protein separation and purification.

Key words: Molecular imprinting, Molecular recognition, Bovine hemoglobin, Photografting, Iniferter

CLC Number:

O657.32
O643

TrendMD:

GAI Qing-Qing^1,3, LIU Qiu-Ye¹, LI Wen-You¹, HE Xi-Wen¹*, CHEN Lang-Xing¹, ZHANG Yu-Kui^1,2*. Preparation of Bovine Hemoglobin-imprinted Polymer Beads via Photografting Surface-Modified Method[J]. Chem. J. Chinese Universities, doi: .

[1]	WANG Longjie, FAN Hongchuan, QIN Yu, CAO Qiue, ZHENG Liyan. Research Progress of Metal-organic Frameworks in the Field of Chemical Separation and Analysis [J]. Chem. J. Chinese Universities, 2021, 42(4): 1167.
[2]	ZHANG Xiaorong, CHEN Lanlan, HU Shanwen. Advances in Bacteria Biosensing Based on Molecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3468.
[3]	JI Cailing, CHENG Xing, TAN Jie, YUAN Quan. Selection of Functionalized Aptamers and Their Applications in Molecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3457.
[4]	LIU Xuejiao, YANG Fan, LIU Shuang, ZHANG Chunjuan, LIU Qiaoling. Progress in Aptamer-targeted Membrane Protein Recognition and Functional Regulation [J]. Chem. J. Chinese Universities, 2021, 42(11): 3277.
[5]	LIU Yuan, DENG Jinqi, ZHAO Shuai, TIAN Fei, LI Yi, SUN Jiashu, LIU Chao. Lateral Flow Assay Based on Molecular Recognition for Diagnosis of Corona Virus Disease 2019 Infection [J]. Chem. J. Chinese Universities, 2021, 42(11): 3390.
[6]	LIN Ningqin, YAO Ke, CHEN Xiangjun. Research Progress of Molecular Recognition and Interaction of Crystallins Linking Cataract [J]. Chem. J. Chinese Universities, 2021, 42(11): 3379.
[7]	HUANG Ling, ZHUANG Zijian, LI Xiang, SHI Muling, LIU Gaoqiang. Advances in Molecular Recognition of Exosomes Based on Aptamers [J]. Chem. J. Chinese Universities, 2021, 42(11): 3493.
[8]	WANG Xiaoru,ZHANG Na,XING Jun. Preparation and Application of Melamine Imprinted Material Using Itaconic Acid as Multidentate Functional Monomer [J]. Chem. J. Chinese Universities, 2020, 41(7): 1521.
[9]	LI Ying, KANG Junjun, ZHAO Xueru, XU Wenkai, QI Qi. Preparation of Gold-modified Magnetic Graphene-based Molecularly Imprinted Composites and Electrochemical Sensing Detection of Dinbutyl Phthalate in Water^† [J]. Chem. J. Chinese Universities, 2019, 40(3): 448.
[10]	MA Yucong,FAN Baomin,HAO Hua,LÜ Jinyu,FENG Yunhao,YANG Biao. Experimental and Theoretical Studies of Action Mechanism of an Octadecylamine-based Molecular Assembly on Mild Steel^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 96.
[11]	MENG Zihui,WANG Yifei,XIE Tengsheng,CHEN Wei,QIU Lili,XUE Min. Molecularly Imprinted Hollow Spheres for the Solid Phase Extraction of Protein^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 62.
[12]	XU Wanzhen, QIU Chunxiao, HUANG Weihong, LIU Hong, YANG Wenming. Computer Simulation Design, Preparation and Application of Fluorescence Sensors Based on Quantum Dots for Selective Detection of 4-Nitrophenol in River Water^† [J]. Chem. J. Chinese Universities, 2017, 38(7): 1155.
[13]	SHEN Xiaoqin, LI Zhi, WANG Ganglin, WANG Li, SUN Quanhong, LUO Xucheng, MA Nan. Logic and Reversible Dual DNA Detection Based on the Assembly of Graphene Oxide and DNA-templated Quantum Dots^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2176.
[14]	SONG Chunxia, YANG Xiaohai, WANG Kemin, WANG Qing, LIU Jianbo, HUANG Jin, LI Wenshan, HUANG Haihua, LIU Wei. Application of Polymers in Fluorescence Analysis^† [J]. Chem. J. Chinese Universities, 2016, 37(2): 201.
[15]	WU Chaochao, LIU Gen, XU Junli, ZHANG Xia. Synthesis of PVDF/ZrO₂ Hybrid Membranes and Its Adsorption Property Toward Bovine Hemoglobin^† [J]. Chem. J. Chinese Universities, 2014, 35(4): 784.

Preparation of Bovine Hemoglobin-imprinted Polymer Beads via Photografting Surface-Modified Method

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments