Quartz Crystal Microbalance Immunosensor for Schistosoma-japonicum

Abstract

Abstract: Aquartz crystal microbalance(QCM) immunosensor has been developed for the direct determination of schistosoma-japonicum-infected rabbit serum(Sj-IRS). Self-assembled monolayers(SAMs) with carboxyl groups were first coated on the gold electrodes of the quartz crystal resonators by spontaneous adsorption of mercaptopropionic acid(MPA). Schistosoma-japonicum molecular antigen of 32 kDmolecular weight(SjAg32) was then covalently attached to the crystal surface via EDCand NHS. The results show that the SAM-coated quartz crystal has a better oscillating behavior in aqueous solutions than ordinary polymer coated quartz crystal. The QCMimmunosensor was used for the detection of infected rabbit serum(IRS49-2000), and a maximum titer of 1:1500 was achieved. Finally, several rabbit serum samples with different degrees of infection were tested. The results show that the QCMimmunosensor is suitable for detecting the infection degree of IRS.

Key words: QCM immunosensor, Self-assembled monolayer, Mercaptopropionic acid, Schistosoma-japonicum

CLC Number:

O657

TrendMD:

WU Zhao-Yang, HE Chun-Ping, WANG Shi-Ping, SHEN Guo-Li, YU Ru-Qin . Quartz Crystal Microbalance Immunosensor for Schistosoma-japonicum[J]. Chem. J. Chinese Universities, 2001, 22(4): 542.

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Quartz Crystal Microbalance Immunosensor for Schistosoma-japonicum

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