Abstract: Prostate cancer is a common malignancy in men. For prostate dependent on androgen for growth, orchiectomy is adopted in clinical therapy along with medicines aimed to restrain the synthesis and secretion of androgens in adrenal. The function of adrenal androgen is not clear till now, therefore, it needs more consideration on how to operate the therapy properly. We established a GC/MRM method for the detection of androgens in tissue sample, by which we monitored the concentration of androgens in emasculated rat. It is shown that the influence of adrenal androgen on the hormone metabolism in prostate existed via this experiment.

Key words: Prostate, Androgen, Gas chromatography-Mass spectrometry