The Interaction of Vanadyl Ions with G-Actin

Abstract

Abstract: The interaction of VO²⁺ and G-actin was studied by gel-chromatography, fluorescence and CDspectroscopies.The results revealed that for one G-actin molecule, there is one strong-binding site and several weak-binding sites for VO²⁺, In low molar ratio of VO²⁺; G-actin vanadyl ions occupy the strong-binding site at first and cause an increase of α-helix content and transformation of the conformation to a more compact one.As the molar ratio increases, vanadyl ions bind to weak-binding sites, but the α-helix content decreases and the conformation becomes rather opened.By measuring light-scattering data as the function of time, the kinetics of G-actin association was studied.In a low concentration, the VO²⁺ ions, like Mg²⁺, promote the association, but in case of higher concentration, the effect turns to be inhibitive.However, the nature of linear association is not changed.

Key words: Vanadyl ions(Vo²⁺), G-actin, Conformation, Association

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AN Fang, ZHANG Bo-Yan, CNEN Bao-Wei, WANG Kui. The Interaction of Vanadyl Ions with G-Actin[J]. Chem. J. Chinese Universities, 1996, 17(5): 667.

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The Interaction of Vanadyl Ions with G-Actin

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