Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer

Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (4): 544.

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Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer

ZHU Qing-Zhi¹, YANG Huang-Hao¹, LI Dong-Hui², XU Jin-Gou¹, ZHANG Yi¹, ZHANG Chang-Gong²

1. The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Department of Chemistry, Xiamen University, Xiamen, 361005;
2. The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry Cancer Research Center, Xiamen University, Xiamen, 361005

Received:1998-10-28 Online:1999-04-24 Published:1999-04-24

Abstract

Abstract: Anovel fluorescence immunoassay for HBsAg was developed by using poly-N-iso-propylacrylamide(PNIP) as an immunoreaction carrier. In sandwich immunoassay, after two steps immunoreaction, PNIP precipitates out of water above a critical temperature of 31℃ , enabling a polymer-bound immune complex to be separated from the solution. The calibration graph for HBsAg was linear over the range of 0.5-100 g/mLwith a detection limit of 10ng/mL. This method combines some advantages of both homogeneous and heterogeneous immunoassays, and has been applied to determining the HBsAg in human blood serum with satisfactory results.

Key words: Poly-N-iso-propylacrylamide, Thermal phase separating technique, Fluorescence immunoassay, HBsAg

CLC Number:

O657.3

TrendMD:

ZHU Qing-Zhi, YANG Huang-Hao, LI Dong-Hui, XU Jin-Gou, ZHANG Yi, ZHANG Chang-Gong . Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer[J]. Chem. J. Chinese Universities, 1999, 20(4): 544.

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Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer

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