CJCU

Chem. J. Chinese Universities

• 研究论文 • Previous Articles     Next Articles

Synthesis, Characterization and Binding of Tea Polyphenol-Manganese and Apoptosis Rate of Induced Tumor Cells

HUANG He-Ning1,3, LI An-Zhang1, WENG Lu-Na1, LIN Qing-Mei2, HUANG He-Qing1,2*, ZHENG Zhong-Hui1, LI Qi-Fu1, CAI Zong-Wei4   

    1. The Key Laboratory of MOE for Cell Biology and Tumor Cell Engineering, School of Life Sciences,
    2. College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China;
    3. Department of Chemical and Biological Engineering, College of Sanming, Sanming 365004, China;
    4. Department of Chemistry, Hong Kong Baptist University, Hong Kong
  • Received:2006-06-19 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10
  • Contact: HUANG He-Qing

PDF (PC)

Cited

11

Abstract: Both tea polyphenol-manganese(TP-Mn) and germanium(TP-Ge) were synthesized with organic synthesis approaches. The characterizations of TP-Mn were clearly revealed via infrared spectrometry and HPLC-ESI-Q-TOF mass spectrometry, respectively. Both HPLC and ICP-MS methods were employed to study the capacities for bovine serum albumen(BSA) binding to TP-Mn, indicating these capacities feasibility. Apoptosis rates in Hela ovarian induced with TP and TP-Mn were studied by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenly tetrazolium bromide(MTT) method. The experimental results show that both TP and TP-Mn can induced the cancer cell apoptosis, but the apoptosis rate with TP-Mn showed two times higher than that with TP. As compared experiments, the same apoptosis rates, going with up to approximately 86%, in Raji human B lymphoma induced with both TP and TP-Mn were observed directly. Human serum albumen might be employed to be a pharmacy carrier for transferring TP-Mn and TP-Ge in vivo.

Key words: Tea polyphenol-Mn, Tea polyphenol-Ge, Tumor cell, Apoptosis rate, ICP-MS

CLC Number: 

TrendMD: