Abstract: High-resolution two-dimensional polyacrylamide gel electrophoresis(2DE), followed by computer assisted image analysis was used to screen protein patterns of normal and infarcted cardiac muscle tissue for quantitative and qualitative differences in protein expression. In the gels of pH=5—8 IPG strips, 851 protein spots were detected in normal cardiac muscle tissues and 1 032 protein spots were resolved in infarcted cardiac muscle tissues. Thirteen protein spots were only expressed in normal cardiac muscle tissues, and 14 protein spots were only expressed in infarcted cardiac muscle tissues. The results also showed that 49 protein spots displayed quantitative changes in expression between normal and infarcted cardiac muscle tissue. Eleven protein spots were subjected to MS analysis and 7 proteins were identified by PMF. These proteins may be involved in the cardiovascular injury, and play an important role for the future treatment of coronary heart disease.

Key words: Myocardial infarct, Cardiac muscle tissue, Proteome, Two-dimensional gel electrophoresis