Preparation of Multi-layer Open Capillary Immobilized Enzyme Reactor and Its Application in Digestion of Proteins

doi:10.3969/j.issn.0251-0790.2012.02.010

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (02): 268.doi: 10.3969/j.issn.0251-0790.2012.02.010

• Analytical Chemistry • Previous Articles Next Articles

Preparation of Multi-layer Open Capillary Immobilized Enzyme Reactor and Its Application in Digestion of Proteins

HU Jia-Wei¹, SHEN Ye-Hua¹, QIN Wei-Jie², ZHANG Yang-Jun², Qian Xiao-Hong²

1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Drug Research Institute, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China;
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China

Received:2011-02-07 Online:2012-02-10 Published:2012-01-13

Abstract

Abstract: With the capillary as the carrier of enzyme immobilization, poly(amidoamine) was gradually bon-ded to the inner wall of the capillary, then the trypsin was attached at the amino group of poly(amidoamine) with glutaraldehyde, and finally the multi-layer open capillary immobilized enzyme reactor was prepared after enzyme immobilization conditions had been optimized. Cyt-c was chosen as a model protein for evaluation of the performance of the multi-layer open capillary immobilized enzyme reactor, and its digestion conditions were optimized. The results demonstrate that the multi-layer immobilized enzyme reactor has high efficiency, good reproducibility and stability, and can be further applied in future proteomics study.

Key words: Open-tubular capillary, Immobilized enzyme reactor, Protein digestion, Dendritical macromolecule

CLC Number:

O657

TrendMD:

HU Jia-Wei, SHEN Ye-Hua, QIN Wei-Jie, ZHANG Yang-Jun, Qian Xiao-Hong. Preparation of Multi-layer Open Capillary Immobilized Enzyme Reactor and Its Application in Digestion of Proteins[J]. Chem. J. Chinese Universities, 2012, 33(02): 268.

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Preparation of Multi-layer Open Capillary Immobilized Enzyme Reactor and Its Application in Digestion of Proteins

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