Preparation and Characterization of PLGA Nanospheres Surface Modified with Biotinylated Chitosan

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (8): 1682.

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Preparation and Characterization of PLGA Nanospheres Surface Modified with Biotinylated Chitosan

CHEN Hong-Li^1*, TANG Hong-Bo², YANG Wen-Zhi², CHEN Han², WANG Yin-Song³, MEI Lin^4,5, ZHANG Tong², XIONG Qing-Qing², ZHANG Qi-Qing^2*

1. Henan Key Laboratory of Hereditary Disease and Molecular Target Drug Therapy(Cultivating Base), Department of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China;
2. Institute of Biomedical Engineering, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China;
3. College of Pharmacy, Tianjin Medical University, Tianjin 300070, China;
4. The Shenzhen Key Lab of Gene and Antibody Therapy, Center for Biotech and Bio-Medicine and Division of Life Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China;
5. College of Pharmacy, Dalian Medical University, Dalian 116027, China

Received:2009-11-05 Online:2010-08-10 Published:2010-08-10
Contact: CHEN Hong-Li, E-mail: chenhlhl@126.com; ZHANG Qi-Qing, E-mail: zhangqiq@xmu.edu.cn
Supported by:
国家重大科学研究计划项目(批准号: 2006CB933300)和新乡医学院省级重点学科开放课题资助.

Abstract

Abstract: Biotinylated chitosan(Bio-CS conjugates or Bio-CS) were synthesized and characterized. The degree of substitution(DS), as defined as the number of biotin per 100 anhydroglucose units of CS, was determined by ¹H NMR and ICP. Poly(lactic-co-glycolic acid)(PLGA) nanospheres were prepared by a solvent evaporation technique(W₁/O/W₂). The surface of PLGA nanosphere was modified with Bio-CS by covalent binding. PLGA nanospheres were almost spherical in shape under the scanning electron microscopy(SEM) observation, and their mean diameter determined by the laser light scattering technique was (248.4±21.0) nm. Bio-CS modified PLGA nanospheres were also spherical in shape; their mean diameter and Zeta potential value were (268.3±23.4) nm and -(21.21±2.13) mV, respectively. The surface chemistry of nanospheres was quantitatively studied by X-ray photoelectron spectroscopy(XPS). The results show the N region corresponding to the primary amide of CS and the S region corresponding to the biotin, indicating that PLGA nanospheres were successfully surface-modified with Bio-CS. The content of biotin on the surface of Bio-CS modified PLGA nanospheres with DS of biotin of 31% was determined using a commercially available Quant*TagTM Biotin Kit and its value was (1.36±0.34) mol/100 mg.

Key words: Nanoparticle, Poly(lactic-co-glycolic acid)(PLGA), Biotinylated chitosan anticancer

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CHEN Hong-Li*, TANG Hong-Bo, YANG Wen-Zhi, CHEN Han, WANG Yin-Song, MEI Lin, .... Preparation and Characterization of PLGA Nanospheres Surface Modified with Biotinylated Chitosan[J]. Chem. J. Chinese Universities, 2010, 31(8): 1682.

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Preparation and Characterization of PLGA Nanospheres Surface Modified with Biotinylated Chitosan

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