Synthesis of COF-LZU1 in Acetate Buffer and Immobilized Enzyme Study

doi:10.7503/cjcu20240368

Abstract

Abstract:

COF-LZU1 was synthesized in acetate buffer at room temperature and used to immobilize laccase and horseradish peroxidase. Firstly， by optimizing the reaction conditions such as reaction concentration， reaction time， pH value of the acetate buffer， reaction temperature， washing solvent and drying method. In acetate buffer， COF-LZU1 was synthesized with high crystallinity at pH=4.5 with stirring at room temperature for 30 min， which has high specific surface area up to 501 m²/g， and higher thermal stability（480 ℃）. Then， under the aforementioned optimal reaction conditions， laccase and horseradish peroxidase were immobilized by in situ embedding with COF-LZU1 as a carrier， and their properties were investigated， which showed that the enzyme activities after immobilization were as high as 84.26% and 73.66%（with respect to the free enzyme activity）， and the relative activities were still maintained at about 80% after cycling for 10 times of use. Through multiple binding sites， COF-LZU1 effectively stabilized the active conformation of the enzyme， which made it less prone to structural deformation and improved the thermal stability， pH stability and reusability of the enzyme， etc. Acetate buffer is a commonly used buffer in biochemical experiments， which was used as both a solvent and a catalyst in the present experiments. Compared with the existing synthetic methods， the method has a better effect on the stability of biomolecules and is expected to provide a new solution for enzyme immobilization.

Key words: COF-LZU1, Acetate buffer, Room temperature, Immobilized enzyme

CLC Number:

O621.3

TrendMD:

LIU Meng, XU Yi, YANG Fan, ZHOU Quan, REN Jing, REN Ruipeng, LYU Yongkang. Synthesis of COF-LZU1 in Acetate Buffer and Immobilized Enzyme Study[J]. Chem. J. Chinese Universities, 2025, 46(2): 20240368.

Figures/Tables 5

References 28

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