Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (8): 1581.

• Articles • Previous Articles     Next Articles

Interaction Between PVA and Staphylokinase and Its Influence on the Secondary Structure of Staphylokinase

WANG Gai-Zhen2, HE Jin-Tian1*, ZHOU Zhi-Tao1, ZHU Yu-Kun1, GUO Yin-Yin2   

  1. 1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;
    2. College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
  • Received:2008-12-30 Online:2009-08-10 Published:2009-08-10
  • Contact: HE Jin-Tian. E-mail: he_jintian@sina.com
  • Supported by:

    河北师范大学应用开发基金(批准号: 2007K05)资助.

Abstract:

Gel filtration chromatography, fluorescence spectrophotometry, differential scanning calorimetry(DSC), Fourier transform infrared spectrophotometry(FTIR) and fibrinolytic activity analysis were used to investigate the interaction between polyvinyl alcohol(PVA) and staphylokinase under physiological conditions. The results show that staphylokinase can form complex with PVA. The results of fluorescence spectrophotometry and DSC suggested that the interaction of PVA with staphylokinase did not destroy advanced structures of staphylokinase. FTIR was combined with resolution enhancement technique Fourier deconvolution and Gaus-sian curve-fitting procedures to quantitate the spectral information from the amide Ⅰ bands of staphylokinase within the freeze-dried mixture of PVA and staphylokinase. The results show that the interaction of PVA with staphylokinase decrease β-sheet content which is associated with intermolecular interaction and protein denaturation. Analysis of fibrinolytic activity of the staphylokinase show that interaction of PVA with the staphylokinase do not destroy the tertiary structure of the staphylokinase.

Key words: Polyvinyl alcohol; Staphylokinase; Fourier transform infrared spectrometry(FTIR); Gel chromatography; Fluorescence spectrometry

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