Influence of DOPC, DOPE and Ceramide on the Organization of Sphingomyelin/Cholesterol Bilayers

Abstract

Abstract:

Lipid rafts are of a dynamic microdomain structure found in recent years, enriched in signal molecules. The change of structure and function of lipid rafts can result in many diseases. LB technique and atomic force microscopy were used to study the effect of 1,2-dioleoy-sn-glycero-3-phosphocholine(DOPC), 1,2-dioleoy-sn-glycero-3-phosphoethanolamine(DOPE) and ceramide on the structure of sphingomyelin(SM)/Cholesterol LB bilayers. We found that each bilayer of lipid mixtures took on uniform distribution bilayer structure when the surface pressure was low. When increasing the surface pressure, great changes were taken place on the bilayers: (1) The bilayers composed of SM/Chol appeared uniform liquid-ordered microdomains structure, with coverage of 80% of mica. (2) In SM/Chol/DOPC bilayers, the liquid-ordered microdomains formed by SM/Chol floated onto the fluid-disordered small DOPC granule, about 30% coverage. (3) When adding DOPE to SM/Chol, the bilayers had changed obviously, meanwhile liquid-disordered, liquid-ordered and gel phases coexisted on the mica. (4) The Ceramide induced the bilayers of SM/Chol rearrangement, the lipids of bilayers occurred flip-flop to form vesicle structure. At the higher surface pressure, all the systems could form specific platform structure. The bond capabilities of molecular groups play an important role in forming structure.

Key words: Lipid raft, LB technique, Atomic force microscopy(AFM), Phase separation

TrendMD:

HAO Chang-Chun, SUN Run-Guang*, ZHANG Jing. Influence of DOPC, DOPE and Ceramide on the Organization of Sphingomyelin/Cholesterol Bilayers[J]. Chem. J. Chinese Universities, 2009, 30(5): 913.

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