Soy Protein Isolate/Agar Composite Hydrogel†

doi:10.7503/cjcu20170450

Abstract

Abstract:

Agar, a plant polysaccharide, was selected to form composite hydrogel with pure and polyethylene glycol(PEG)-modified soy protein isolates(SPI) by a simple blending method. It was found that SPI/agar mixture was in solution state at high temperature but quickly formed a hydrogel after cooling down to the physiological temperature(37 ℃) or lower. Therefore, such a natural polymer-based thermo-sensitive hydrogel can be designed into different shapes. SEM images indicated that PEG-modified SPI/agar hydrogel had more compact network structure than the pure SPI/agar hydrogel. The swelling ratio, mechanical properties, cytotoxicity, and cell migration ability of both pure and PEG-modified SPI/agar composite hydrogel were extensively investigated. Compared with the pure agar hydrogel and pure SPI/agar composite hydrogel, PEG-modified SPI/agar hydrogel showed the largest swelling ratio(higher than 1600%). In addition, the PEG-modified SPI/agar composite hydrogels showed good mechanical properties, which was comparable to that of pure agar hydrogel. In vitro cell experiments demonstrated that PEG-modified SPI/agar hydrogel had both good biocompatibility and high cell migration ability. Therefore, all excellent properties shown above made the PEG-modified SPI/agar hydrogel a good candidate for wound dressing.

Key words: Polyethylene glycol-modified soy protein isolate, Agar, Composite hydrogel, Wound dressing

CLC Number:

O636

TrendMD:

LIU Jie, ZHOU Hao, HUANG Yufang, CHEN Xin. Soy Protein Isolate/Agar Composite Hydrogel^†[J]. Chem. J. Chinese Universities, 2018, 39(3): 591.

Figures/Tables 6

Fig.1 Optical images of pure agar hydrogel(a) and PEG modified SPI/agar hydrogel(b)(A) and different shaped PEG modified SPI/agar hydrogel(blue color comes from methylene blue)(B)

Fig.2 SEM images of lyophilized pure SPI/agar(A) and PEG modified SPI/agar hydrogels(B)

Fig.3 Swelling ratio of pure agar(a), pure SPI/agar(b), SPI-g-mPEG/Aga20∶1(c) and SPI-g-mPEG/Aga10∶1(d) with different agar contents

Fig.4 Compression curves(A), compressive stress(B), compressive strain(C) and compressive modulus(D) of pure agar(a), pure SPI/agar(b), SPI-g-mPEG/Aga20∶1(c) and SPI-g-mPEG/Aga10∶1(d)(A) a. Aga0.5%; b. Aga0.5%/SPI(5%); c. Aga0.5%/SPI-g-mPEG10∶1(5%); d. Aga0.5%/SPI-g-mPEG20∶1(5%).

Fig.5 Cell viability of PEG modified SPI/agar hydrogels with different agar contents for L929 cells via CCK-8 assayPEG modified SPI sample: SPI-g-mPEG/Aga 10∶1. Content of leaching liquor: a. 20%; b. 50%; c. 75%; d. 100%.

Fig.6 L929 cell migration with pure agar(A1—A3), pure SPI/agar(B1—B3) and SPI-g-mPEG/Aga 10∶1 hydrogels(C1—C3)t/h: (A1—C1) 0; (A2—C2) 24; (A3—C3) 48.

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