Antibacterial Indole Trimer from Metagenomic Library of Marine Sponge Discodermia calyx

doi:10.7503/cjcu20130275

Abstract

Abstract:

Marine sponge Discodermia calyx harboring highly complex consortia is a rich and important source of biologically active compounds. Considering the chemical and biological diversity present in the sponge microbial community, the metagenomic library of marine sponges should be an interesting resource to search for unique bioactive small molecules and the corresponding unknown functional genes. Therefore, we constructed the metagenomic library of a marine sponge, Discodermia calyx. Functional screening of this library afforded one antibacterial clone pDC111 by two-layer top agar method. The antibacterial clone pDC111 was cultured and the chemical constituent was analyzed and separated by bioassay-guided fractionation. Compound 1 was afforded and identified as indole trimer by its 1D NMR(¹H NMR, ¹³C NMR), 2D NMR(¹H-¹H COSY, HMQC, HMBC) and HR-TOFMS data. Antibacterial test results showed that compound 1 exhibited anti-Bacillus cereus activity with inhibition zone of 12 mm at 10 μg/paper(i.d. 6 mm). This indicated that functional metagenomics could be used to search for active compounds from marine sponges consisting of large population of uncultured microbes. Furthermore, there are highly potentials that the functional genes of indole trimer 1 could be identified by further molecular biology techniques investigation.

Key words: Marine sponge, Metagenomic library, Two-layer top agar, Indole trimer

CLC Number:

TrendMD:

HE Rui, WANG Bo-Chu, ZHU Lian-Cai, LIU De-Fang. Antibacterial Indole Trimer from Metagenomic Library of Marine Sponge Discodermia calyx[J]. Chem. J. Chinese Universities, 2013, 34(12): 2772.

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