Preparation of High-Performance QD-encoded Magnetic Barcodes†

doi:10.7503/cjcu20160788

Abstract

Abstract:

The key element of the suspension array based multiplexed assays lies in the preparation of barcodes. Quantum dots(QDs) are ideal candidate fluorophores for optical encoding because of their outstanding fluorescent properties. By using chloroform/butanol mixed solution as CdSSe/ZnS QD reaction solvent, hydrophobic CdSSe/ZnS QDs were loaded onto the surface of magnetic polystyrene spheres via the modified layer-by-layer assembly proposed here. By the adjustment of QD concentration, high-performance CdSSe/ZnS QD encoded magnetic barcodes with different fluorescence intensity levels were successfully prepared. By comparing the CdSSe/ZnS QD encoded magnetic barcodes respectively prepared by using chloroform and chloroform/butanol mixed solution as reaction solvent, it was testified that the mixed solution helped to avoid the good QD solvent’s damage to the morphology of polymer spheres and facilitated the assembly of CdSSe/ZnS QDs. The prepared CdSSe/ZnS QD encoded magnetic barcodes demonstrated good dispersity in water, narrow distribution of fluorescence intensity and uniform morphology. This method paves a new way for simple, accurate and controllable preparation of QD-encoded barcodes with high encoding capacity.

Key words: Quantum dots, Fluorescence, Barcode, Layer-by-layer assembly, CdSSe, ZnS, Magnetic polystyrene

CLC Number:

O614

TrendMD:

LU Si, ZHANG Dingshengzi, LIU Bing, XU Hong, GU Hongchen. Preparation of High-Performance QD-encoded Magnetic Barcodes^†[J]. Chem. J. Chinese Universities, 2017, 38(4): 509.

Figures/Tables 9

Scheme 1 Fabrication of CdSSe/ZnS QD encoded magnetic barcodes

Fig.1 Zeta-potential of the microspheres at corresponding assembly stages(A), kinetics curve of CdSSe/ZnS QD loading process during the preparation of M-CdSSe/ZnS-MBs(B) and confocal image of M-CdSSe/ZnS-MBs(C)

Fig.2 SEM images of C-CdSSe/ZnS-MBs(A) and M-CdSSe/ZnS-MBs(B)Insets: confocal images of (A) and (B).

Fig.3 Scatter plots(A, C) and histogram(B, D) of C-CdSSe/ZnS-MBs(A, B) and M-CdSSe/ZnS-MBs(C, D) via flow cytometry

Fig.4 SEM images(A—E) and fluorescence intensity(F) of M-CdSSe/ZnS-MBs prepared by using CHCl3/C4H9OH in different volume ratios VCHCl3)/VC4H9OH): (A) 2∶1; (B) 1∶1; (C) 1∶2; (D) 1∶5; (E) 1∶10.

Fig.5 Relationship between the fluorescence intensity of M-CdSSe/ZnS-MBs and the concentration of the first layer PEI(A) and the concentration of the second layer PEI(B)

Fig.6 Emission spectra of M-CdSSe/ZnS-MBs with fluorescence intensity levels of L1—L6Inset: Enlarge emission spectra of L1—L3.

Fig.7 Scatter plots and confocal images(insets) of L1(A), L2(B), L3(C), L4(D), L5(E), L6(F), respectively

Fig.8 Flow cytometry analysis(A—C) of L1—L6 mixed together: scatter plot of FSC and SSC signal(A), histogram of L1—L6 distinguished by fluorescence intensity(B), scatter plot of L1—L6 distinguished by fluorescence intensity(C)

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