Rheological Properties of GuHCl/Type Ⅰ Collagen Dispersions†

doi:10.7503/cjcu20160063

Abstract

Abstract:

Collagen was extracted from the outer skin of pig using a pepsin digestion method. The isolated collagen was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), Fourier transform infrared spectroscopy(FTIR) and ultraviolet(UV). According to the electrophoretic pattern, the isolated collagen consisted of two different α-chains(α₁, α₂) and one β-chain, indicating type Ⅰ collagen. FTIR and UV results confirmed the presence of triple helical structure in the collagen molecule. The rheological properties of GuHCl/type Ⅰ collagen dispersions were investigated as a function of guanidine hydrochloride(GuHCl) concentration and action time. The results of flow curves indicated that the pseudoplastic flow behavior of GuHCl/collagen dispersions gradually close to the Newtonian fluid with the increase of GuHCl concentration in the range of 0—6.0 mol/L and action time in the range of 6—48 h. The linear oscillatory shear, steady shear and thixotropic loop experiments were performed to investigate thixotropic behavior of the GuHCl/collagen dispersions. It was found that the thixotropic type of GuHCl/collagen dispersions transformed from positive thixotropy to complex thixotropy and then to negative thixotropy with the increase of GuHCl concentration. The thixotropic type of collagen dispersion transformed from complex thixotropy to negative thixotropy with the increase of action time of 1.0 mol/L GuHCl. It should be note that the complex thixotropy in GuHCl/collagen dispersions has not been reported in the similar systems so far. And we tentatively discussed this novel discovery on the basis of the interactions between GuHCl molecule and collagen. Meanwhile, this work would be helpful to deeply understand the mechanism of thixotropy.

Key words: Collagen, GuHCl, Denaturation, Rheological property, Complex thixotropy

CLC Number:

O631

TrendMD:

BU Honghong, JIAO Yanni, LAN Wenjun, ZHUANG Xiaoli, DAI Xiaonan, LI Tianduo. Rheological Properties of GuHCl/Type Ⅰ Collagen Dispersions^†[J]. Chem. J. Chinese Universities, 2016, 37(6): 1189.

Figures/Tables 12

Fig.1 Protein patterns(A), FTIR(B) and UV-Vis(C) spectra of pig skin collagenLane 1 represents the protein molecular weight marker; Lane 2, 3 represent pig skin collagen.

Fig.2 SEM(A—D)and TEM(E, F) images of GuHCl/collagen dispersions(A) 0.1 mg/mL collagen; (B) 0.1 mg/mL collagen+0.5 mol/L GuHCl; (C) 0.1 mg/mL collagen+1.0 mol/L GuHCl;(D) 0.1 mg/mL collagen+4.0 mol/L GuHCl; (E) 0.1 mg/mL collagen; (F) 0.1 mg/mL collagen+4.0 mol/L GuHCl.

Fig.3 Flow curves of GuHCl/collagen dispersions at different GuHCl concentrations

Table 1 Power-law equation and R2 of GuHCl/collagen dispersions at different GuHCl concentrations

System	Power-law equation	R²
Collagen	τ=0.544 D^0.49	0.9957
Collagen+1.0 mol/L GuHCl	τ=0.073 D^0.68	0.9952
Collagen+2.0 mol/L GuHCl	τ=0.003 D^0.92	0.9961
Collagen+4.0 mol/L GuHCl	τ=0.002 D^0.95	0.9936
Collagen+6.0 mol/L GuHCl	τ=0.001 D^0.96	0.9967

Fig.4 Effect of 1.0 mol/L GuHCl function time on flow curves of collagen dispersionsTime/h: a. 6; b. 12; c. 24; d. 48.

Table 2 Power-law equation and R2 of GuHCl/collagen dispersions at different GuHCl(1.0 mol/L) function time

Time/h	Power-law equation	R²
6	τ=0.128 D^0.61	0.9946
12	τ=0.073 D^0.68	0.9952
24	τ=0.045 D^0.77	0.9969
48	τ=0.002 D^0.95	0.9980

Fig.5 Effect of GuHCl concentration on the thixotropy of collagen dispersions under oscillatory shear experimentsc(GuHCl)/(mol·L-1): (A) a. 0.25; b. 0.5; c. 0.75; d. 0. (B) a. 1.0; b. 2.0; c. 4.0; d. 6.0.

Fig.6 Effect of GuHCl function time on the thixotropy of collagen dispersions under oscillatory shear experiments

Fig.7 Thixotropy of collagen dispersions with different concertrations of GuHCl under steady shear experiments

Fig.8 Thixotropy of collagen dispersions with different function time of GuHCl under steady shear experiments

Fig.9 Thixotropic loop of collagen dispersions with different c(GuHCl)

Fig.10 Thixotropic loop of collagen dispersions with different function time of GuHCl(1.0 mol/L)Time/h: a. 6; b. 12; c. 24; d. 48.

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