Probing Protein Functions via Optical Single Molecule Detection

Chem. J. Chinese Universities

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Probing Protein Functions via Optical Single Molecule Detection

LI Xun, ZHAO Xin-Sheng*

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

Received:2008-09-17 Revised:1900-01-01 Online:2008-12-10 Published:2008-12-10
Contact: ZHAO Xin-Sheng

Abstract

Abstract: In the last two decades, optical single molecule detection(SMD) has been rapidly developed as a powerful tool in many research fields, especially, in biological systems. This mini-review focuses on the application of SMD in protein studies. Based on the unique advantage, namely providing detailed distribution of molecular properties and tracking individual molecules in real time, SMD is applied to not only equilibrium yet heterogeneous systems such as protein folding, enzyme reaction dynamics, and membrane dynamics, but also non-equilibrium events. SMD plays an essential role in tracking the motion of protein motors in vivo.

Key words: Single molecule detection, Protein, Dynamic

CLC Number:

O657

TrendMD:

LI Xun, ZHAO Xin-Sheng*. Probing Protein Functions via Optical Single Molecule Detection[J]. Chem. J. Chinese Universities, doi: .

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Probing Protein Functions via Optical Single Molecule Detection

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