Fabrication and Characterization of Poly(L-lactic acid)-polycaprolactone Composite Nanofiber Scaffolds†

doi:10.7503/cjcu20140365

Abstract

Abstract:

The feasibility of fabrication of poly(L-lactic acid)-polycaprolactone(PLLA-PCL) composite nanofiber scaffolds using the gel extraction phase separation in the dioxane/ethanol solvent system was verified and the effects of gelation temperature, the ratio of PLLA and PCL, polymer concentration, the ratio of dioxane and ethanal and porogen on the structure and performance of PLLA-PCL composite nanofiber scaffolds were investigated. The results show that PLLA-PCL composite nanofiber scaffolds with 50—500 nm nanofiber structure similar to the extracellular matrixc(ECM) can be fabricated when the gelation temperature is in the range of -20—-10 ℃, PCL content of 30% to 50%, the content of non-solvent no more than 15%, the porogen proportion no more than 1∶1. The additional PCL play a role of toughening, and the elastic modulus of the composite fiber scaffolds decrease with the increasing of PCL mass fraction. When the content of PCL is 30%, the compatibility and crystallinity of the composite fiber scaffolds are the best, and the composite fiber scaffolds have a good bioactivity and certain biodegradability.

Key words: Poly(L-lactic acid), Polycaprolactone, Nanofiber, Scaffold, Phase separation

CLC Number:

TrendMD:

HE Zhihang, LIU Jia, JIAO Jianjin, WU Guifang, XIAO Xiufeng. Fabrication and Characterization of Poly(L-lactic acid)-polycaprolactone Composite Nanofiber Scaffolds^†[J]. Chem. J. Chinese Universities, 2014, 35(10): 2265.

Figures/Tables 10

Fig.1 SEM images of PLLA-PCL scaffolds prepared in dioxane/ethanol(90∶10) at -10 ℃(A) and -20 ℃(B)

Fig.2 SEM images of PLLA-PCL scaffolds prepared in dioxane/ethanol(90∶10, mass ratio) with different mass ratios of PLLA/PCL Mass ratio of PLLA/PCL: (A) 50∶50; (B) 60∶40; (C) 70∶30; (D) 80∶20; (E) 90∶10.

Fig.3 SEM images of PLLA-PCL scaffolds prepared at -10 ℃ with different mass ratios of dioxane/ethanol Mass ratio of dioxane/ethanol: (A) 85∶15; (B) 90∶10; (C) 95∶5.

Fig.4 SEM images of PLLA-PCL scaffolds prepared at -10 ℃ with different mass ratios of porogen Mass ratio of porogen: (A) 0∶1; (B) 10∶1; (C) 20∶1; (D) 30∶1.

Fig.5 DSC curves(A) and XRD patterns(B) of PLLA-PCL scaffolds prepared in dioxane/ethanol (90∶10, mass ratio) with different mass ratios of PLLA/PCL Mass ratio of PLLA/PCL: (A) 90∶10; (B) 80∶20; (C) 70∶30; (D) 60∶40; (E) 50∶50.

Table 1 Porosity of PLLA-PCL composite scaffolds at different fabrication conditions

Mass ratio of PLLA/PCL	Mass ratio of dioxane/ethanol	Mass ratio of porogen/solution	Gelation temperature/℃	Porosity(%)
90∶10	90∶10	0	-10	92.04±0.28
80∶20	90∶10	0	-10	92.53±0.20
70∶30	90∶10	0	-10	93.02±0.33
60∶40	90∶10	0	-10	94.48±0.29
50∶50	90∶10	0	-10	94.76±0.30
70∶30	90∶10	0	-20	93.93±0.29
70∶30	85∶15	0	-10	94.55±0.24
70∶30	95∶5	0	-10	92.39±0.31
70∶30	90∶10	0.5∶1	-10	94.84±0.32
70∶30	90∶10	1∶1	-10	95.75±0.22
70∶30	90∶10	1.5∶1	-10	90.44±0.24

Fig.6 Elastic modulus and porosity of composite scaffolds with different mass ratio of PLLA/PCL

Fig.7 SEM images of PLLA-PCL(70∶30, mass ratio) composite scaffolds with immersion in SBF for 7 d(A) and 14 d(B)

Fig.8 XRD pattern(A) and FTIR spectrum(B) of PLLA-PCL(70∶30, mass ratio) composite scaffolds with immersion in SBF for 14 d

Fig.9 Mass loss rate(a) and pH value(b) of PLLA-PCL composite saffold after different immersion time

References 20

[1]	Hutmacher D. W., Schantz T., Zein I., J. Biomed. Mater. Res. A,2001, 55(2), 203—216
[2]	Woodfield T. B. F., Malda J., Wijn J., Biomaterials,2004, 25(18), 4149—4161
[3]	Sun S. F., Li N. N., Liu M., Liu Y. C., Zhang Y. C., Zhou Y. M., Chem. J. Chinese Universities,2012, 33(12), 2827—2832(孙淑芬, 李男男, 刘敏, 刘译聪, 张英超, 周延民. 高等学校化学学报, 2012, 33(12), 2827—2832)
[4]	Tuli R., Li W. J., Tuan R. S., Arthritis Res. Ther., 2003, 5(5), 235—238
[5]	Hutmacher D. W., Schantz T., Zein I., J. Biomed. Mater. Res. A,2001, 55(2), 203—216
[6]	Xiao X. F., Ding X. H., Liu S. Q., Acta Materiae Compositae Sinica,2010, 27(6), 100—105
	(肖秀峰, 丁晓红, 刘淑琼. 复合材料学报, 2010, 27(6), 100—105)
[7]	Yang J. M., Shyu J. S., Chen H. L., Polym. Eng. Sci., 1997, 37(7), 1182—1187
[8]	Qian H. T., Bei J. Z., Wang S. G., Polym. Degrad. Stab., 2000, 68(3), 423—429
[9]	DellErba R., Groeninckx G., Maglio G., Polymer,2001, 42(18), 7831—7840
[10]	Liu N.H., Miao Y. E., Qi F. Z., Gu J. Y.,Fudan University Journal of Medical Sciences, 2014, (1), 34—39
	(刘宁华, 缪月娥, 亓发芝, 顾建英. 复旦学报, 2014, (1), 34—39)
[11]	Li Y. D., Xu Y., Zhou Q., Song L., Liu W., Gan Y. B., Li P., Li S. T., Mo X. M., J. Third Mil. Med. Univ., 2014, 36(9), 914—918
	(李玉东, 徐源, 周强, 宋磊, 刘威, 甘翼博, 李培, 李松涛, 莫秀梅. 第三军医大学学报, 2014, 36(9), 914—918)
[12]	Langer R., Vacanti J. P., Science,1993, 260, 920—926
[13]	Li H.Q., Zhang C., Polymer Physics, Chemical Industry Press, Beijing, 2007, 94—95
	(励杭泉, 张晨. 聚合物物理学, 北京: 化学工业出版社, 2007, 94—95)
[14]	Ji G. L., Zhu B. K., Cui Z. Y., Polymer,2007, 48(21), 6415—6425
[15]	Ramiro D. E., Gabriel G., Giovanni M., Polymer,2001, 42(18), 7831—7840
[16]	Ren J., Yang Jun., Ren T. B., Polymer Bulletin,2006, 12, 51—56
	(任杰, 杨军, 任天斌. 高分子通报, 2006, 12, 51—56)
[17]	Zhang A. J., J. Shangdong University, 2010, 40(3), 86—90
	(张爱娟. 山东大学学报, 2010, 40(3), 86—90)
[18]	Liu Y., Zhong K. N., Hu W. Y., J. Mater. Sci. Engin., 1997, 15(1), 63—65
	(刘羽, 钟康年, 胡文云. 材料科学与工程学报, 1997, 15(1), 63—65)
[19]	Miyaji F., Iwai M., Kokubo T., J. Mater. Sci. Mater. Med., 1998, 9, 61—65
[20]	Cloing P. G., Zhoung W. G., J. Poly. Sci. A,1991, 24(8), 17360—17365