Effects of Collagen Films with Liquid Crystal-liked Ordered Structure on Adhesion, Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells†

doi:10.7503/cjcu20140336

Abstract

Abstract:

To mimic the ordered arrangement of type Ⅰ collagen in natural tissue, a collagen films with liquid crystal-liked ordered surface were fabricated utilizing the liquid crystal state of concentrated collagen. And the effects of the topography on the behavior of human umbilical cord mesenchymal stem cells(hUCMSCs) were studied in vitro. The results showed that the collagen fibrils arranged directly on the surface of the collagen films when the concentration was over 120 mg/mL. This ordered structure improved significantly the adhesion and proliferation of hUCMSCs. In addition, it also increased the expression of osteogenic gene such as ALP, Collagen Ⅰ , RUNX2, Osterix, OCN and OPN, which meant that hUCMSCs had a stronger differentiation potency on the ordered surface than on the unordered surface.

Key words: Type Ⅰ, collagen, Liquid crystal-liked ordered structure, Human umbilical cord mesenchymal stem cell(hUCMSC), Osteogenic differentiation

TrendMD:

ZENG Qinghui, LI Na, TIAN Ye, WU Di, ZHAO Yaowu, LI Lihua, ZHOU Changren. Effects of Collagen Films with Liquid Crystal-liked Ordered Structure on Adhesion, Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells^†[J]. Chem. J. Chinese Universities, 2014, 35(8): 1658.

Figures/Tables 7

Table 1 Primers for osteogenic related genes

Gene	Primer base sequences(Direction: 5' to 3')	Gene	Primer base sequences(Direction: 5' to 3')
RUNX2 up	AAGTCGGGGGGGAGGTCGTCGG	OPN down	CCTCACTTTTGAAGCCAACGAC
RUNX2 down	TACCACGCCAACAGCACCCCGC	Collagen Ⅰ up	CAGCCGCTTCACCTACAGC
Osterix up	CGGGAGACGCCCTGAGTTGTTG	Collagen Ⅰ down	TTTTGTATTCAATCACTGTCTTGCC
Osterix down	ACCCTTTTCCCTCCCATTAGTA	OCN up	AGAGCGACACCCTAGAC
ALP up	AAGTTTGGCTCTATGTTCGTGA	OCN down	CATGAGAGCCCTCACA
ALP down	CTCTGGGTTATCCATCAGGTGT	GAPDH up	CTCTAGGGAGGTTTTAGTTCAC
OPN up	TGGGAAGGTTCATTCAGGTTGC	GAPDH down	CTACTGGGAAAACCGAGGGGGG

Fig.1 POM images of collagen coatings with different concentrations c/(mg·mL-1): (A) 30; (B) 60; (C) 90; (D) 120.

Fig.2 AFM images of collagen films with unordered surface(A), liquid crystal-liked ordered surface(B) and model image(C) of film cross section in square of (B) c/(mg·mL-1): (A) 30; (B) 120.

Fig.3 OD of hUCMSCs cultured on different surface vs. time * P<0.05 vs. Blank; #: P<0.05, ##: P<0.01.

Fig.4 CLSM images of hUCMSCs cultured on unordered films(A, B) and liquid crystal-liked ordered films(C, D) for 2 d(A, C) and 4 d(B, D)

Fig.5 AFM images of hUCMSCs cultured on unordered films(A, C) and liquid crystal-liked ordered films(B, D) for 2 d with 2D(A, B) and 3D(C, D)

Fig.6 RT-PCR analysis of osteogenic gene expression cultured on collagen film

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