Single-labeled“Turn on” Fluorescent Oligonucleotide Probe for Iodide Detection Based on T-Hg2+-T and Inherent Quenching Ability of G-quadruplex

doi:10.7503/cjcu20120358

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (12): 2651.doi: 10.7503/cjcu20120358

• Analytical Chemistry • Previous Articles Next Articles

Single-labeled“Turn on” Fluorescent Oligonucleotide Probe for Iodide Detection Based on T-Hg²⁺-T and Inherent Quenching Ability of G-quadruplex

ZHU Ying, LIU Pei, YANG Xiao-Hai, HE Lei-Liang, LI Qing-Zhao, WANG Qing, WANG Ke-Min, HUANG Jin, LIU Jian-Bo

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China

Received:2012-04-16 Online:2012-12-10 Published:2012-11-20
Contact: Kemin Wang E-mail:kmwang@hnu.edu.cn;yangxiaohai@hnu.edu.cn

Abstract

Abstract:

A simple and "Turn on" type iodide anion assay method was developed, based on the inherent quenching ability of G-quadruplex and fidelity of the "thymine-Hg²⁺-thymine" binding motif. A T-rich sequence was labeled with 6-carboxyfluorescein(FAM) at its 5'-end, nearby the 3'-end was a G-rich sequence which could form G-quadruplex structure instead of traditional quenchers. Upon addition of Hg²⁺, T-rich sequence folded into a hairpin structure which led the G-quadruplex near to the FAM, and the FAM was quenched by the G-quadruplex owning to photoinduced electron transfer between the dye and the G-quadruplex. After the addition of iodide, the fluorescence of FAM recovered considering that iodide could bind with Hg²⁺. The novel method for the determination of iodide was developed in linear range of 50—500 nmol/L, with the detection limit(3σ) of 30 nmol/L. This proposed method was highly selective and other anions have no interfering effects on the determination, which was successfully applied for analysis of real samples with the recovery from 92%—109%, and the RSD<4%(n=4).

Key words: Iodide, Fluorescence, DNA probe, G-quadruplex

CLC Number:

O657

TrendMD:

ZHU Ying, LIU Pei, YANG Xiao-Hai, HE Lei-Liang, LI Qing-Zhao, WANG Qing, WANG Ke-Min, HUANG Jin, LIU Jian-Bo. Single-labeled“Turn on” Fluorescent Oligonucleotide Probe for Iodide Detection Based on T-Hg²⁺-T and Inherent Quenching Ability of G-quadruplex[J]. Chem. J. Chinese Universities, 2012, 33(12): 2651.

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Single-labeled“Turn on” Fluorescent Oligonucleotide Probe for Iodide Detection Based on T-Hg²⁺-T and Inherent Quenching Ability of G-quadruplex

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