Detection of Hepatitis B Virus DNA Hybridization Based on Dual Gold Nano-probes

doi:10.3969/j.issn.0251-0790.2012.07.010

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (07): 1420.doi: 10.3969/j.issn.0251-0790.2012.07.010

• Analytical Chemistry • Previous Articles Next Articles

Detection of Hepatitis B Virus DNA Hybridization Based on Dual Gold Nano-probes

XING Ya-Si^1,2, ZOU Neng-Li¹, MAO Hong-Ju¹, XU Xia², GE Yu-Qing¹, JIN Qing-Hui¹, ZHAO Jian-Long¹

1. State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, China;
2. College of Pharmacy, Zhengzhou University, Zhengzhou 450001, China

Received:2011-08-22 Online:2012-07-10 Published:2012-07-10

Abstract

Abstract: A novel Hepatitis B virus(HBV) DNA detection method based on dual gold nano-probes and gene chip was developed. Two types of probes including capture probe and reporter probe were designed according to conservative HBV DNA. A pair of complementary gold nanoparticles probes was used to amplify the signal of HBV DNA and visualized by silver staining on gene chip. The optimized detection limit of this method was 10 fmol/L, and the entire test could be completed in 1.5 h. This strategy could be potentially used for HBV DNA detection due to its rapid, high-sensitivity and low-cost properties.

Key words: Dual gold nano-probes, Hybridization, Gene chip, Silver staining, Hepatitis B virus(HBV) DNA

CLC Number:

O657.39

TrendMD:

XING Ya-Si, ZOU Neng-Li, MAO Hong-Ju, XU Xia, GE Yu-Qing, JIN Qing-Hui, ZHAO Jian-Long. Detection of Hepatitis B Virus DNA Hybridization Based on Dual Gold Nano-probes[J]. Chem. J. Chinese Universities, 2012, 33(07): 1420.

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Detection of Hepatitis B Virus DNA Hybridization Based on Dual Gold Nano-probes

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