A Double-Chamber Enzymatic Biofuel Cells-based Self-powered Glucose Biosensor Based on Graphene/Gold Nanoparticles/Titanium Carbide Nanocomposite

doi:10.7503/cjcu20240301

Abstract

Abstract:

Enzymatic biofuel cells（EBFCs）-based self-powered sensing device has the advantages of simple structure， easy miniaturization， and no need for external power supply. It exhibits potential application prospects in clinical diagnosis， environmental monitoring， biosensing， and other fields. Graphene/gold nanoparticles/titanium carbide（rGO/AuNPs/Ti₃C₂） nanocomposite modified glassy carbon electrode（GCE） was used as cathode of EBFCs （rGO/AuNPs/Ti₃C₂/GCE）. The bioanode of EBFCs was prepared via immobilization of glucose oxidase（GOx） on the surface of rGO/AuNPs/Ti₃C₂/GCE. A double-chamber enzymatic biofuel cells-based self-powered glucose biosensor（EBFCs-SPGB） was constructed by combining as-prepared bioanode and cathode in supporting electrolyte separated with a Nafion membrane. In the presence of target glucose， the GOx fixed on the surface of the bioanode promotes an enzymatic reaction. The electrons generated by catalyzing glucose transferred to the cathode through an external circuit， resulting in a reduction reaction on the cathode surface and generating an electrochemical response signal. Due to the excellent conductivity， biocompatibility， and large specific surface area of rGO/AuNPs/Ti₃C₂ nanocomposite， the synergistic effect of nanocomposite can significantly increase loading amount of GOx and effectively promote electron transfer on the electrode surface. The maximum power output signal of the constructed EBFCs-SPGB shows a good linear relationship with glucose concentration in the range of 0.3—10 mmol/L， with a detection limit of 0.1 mmol/L（S/N=3）， which can be applied to analysis of glucose concentration in human serum samples.

Key words: Glucose, Nanocomposite, Enzymatic biofuel cell, Self-powered electrochemical sensor

CLC Number:

O657.1

TrendMD:

LI Shixuan, MENG Hua, YIN Xuehu, YI Jinfei, MA Lihong, ZHANG Yanli, WANG Hongbin, YANG Wenrong, PANG Pengfei. A Double-Chamber Enzymatic Biofuel Cells-based Self-powered Glucose Biosensor Based on Graphene/Gold Nanoparticles/Titanium Carbide Nanocomposite[J]. Chem. J. Chinese Universities, 2024, 45(12): 20240301.

Figures/Tables 7

References 34

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Sample No.	Hospital^*/（mmol·L^-1）	Added/（mmol·L^-1）	Found/（mmol·L^-1）	RSD（%）	Recovery（%）
1	4.90	0	4.86	4.5	—
		0.5	5.41	3.6	102.0
		1.0	5.87	4.6	97.0
		1.5	6.35	3.4	96.6
2	5.19	0	5.20	1.9	—
		0.5	5.70	4.3	102.0
		1.0	6.19	3.6	98.3
		1.5	6.74	3.7	104.0
3	5.25	0	5.24	4.2	—
		0.5	5.77	2.3	104.0
		1.0	6.21	3.0	96.0
		1.5	6.78	3.3	103.0

Sample No.	Hospital^*/（mmol·L^-1）	Added/（mmol·L^-1）	Found/（mmol·L^-1）	RSD（%）	Recovery（%）
1	4.90	0	4.86	4.5	—
		0.5	5.41	3.6	102.0
		1.0	5.87	4.6	97.0
		1.5	6.35	3.4	96.6
2	5.19	0	5.20	1.9	—
		0.5	5.70	4.3	102.0
		1.0	6.19	3.6	98.3
		1.5	6.74	3.7	104.0
3	5.25	0	5.24	4.2	—
		0.5	5.77	2.3	104.0
		1.0	6.21	3.0	96.0
		1.5	6.78	3.3	103.0

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