Silk Fibroin-modified Ploylactic Acid-glycolic Acid Copolymer Porous Microspheres as Gingival Mesenchymal Stem Cells Delivery Carrier †

doi:10.7503/cjcu20190193

Abstract

Abstract:

The polylactic acid-glycolic acid copolymer(PLGA) porous microspheres suitable for three-dimensional cell culture were prepared by double emulsion-solvent evaporation method, which were then subjected to silk fibroin modification, and characterized by scanning electron microscopy, energy dispersive spectroscopy, infrared spectroscopy and X-ray diffraction. Primary cultured human gingival mesenchymal stem cells were used for osteogenic(alizarin red staining) and adipogenic(oil red O staining) differentiation identification experiments. The gingival stem cells were seeded on silk fibroin-modified PLGA porous microspheres by negative pressure suspension method, and the 5-ethynyl-2'-deoxyuridine(EdU) cell proliferation experiment and osteogenic differentiation experiment were performed. The result confirmed that primary cultured gingival stem cells had multi-directional differentiation potential, and those loaded on silk fibroin-modified PLGA porous microspheres were beneficial to cell proliferation. Silk fibroin modified PLGA porous microspheres can be good cell delivery carriers, providing a scientific basis for further repair of alveolar bone defects.

Key words: Gingival mesenchymal stem cell, Polylactic acid-glycolic acid copolymer porous microspheres, Silk fibroin-modification, Tissue repairing

CLC Number:

O631

TrendMD:

LIU Yaoshan,WU Hailin,JIA Zhi,DU Bo,LIU Dayong,ZHOU Zhimin. Silk Fibroin-modified Ploylactic Acid-glycolic Acid Copolymer Porous Microspheres as Gingival Mesenchymal Stem Cells Delivery Carrier ^†[J]. Chem. J. Chinese Universities, 2019, 40(11): 2419.

Figures/Tables 9

Fig.1 SEM images of PLGA porous microspheres without(A―C) and with(D) NaOH treatment PLGA concentration(mg/mL): (A) 16; (B, C, D) 25.

Fig.2 SEM image of PLGA(A) and SEM image(B), EDS(C) and FTIR spectra(D) of SF-PLGA porous microspheres

Fig.3 Optical microscopy images of primary(A) and the third-generation(B―D) gingival mesenchymal stem cells (C) Dyed by alizarin red;(D) dyed by oil red O.

Fig.4 Confocal laser scanning microscopy images of PLGA(A) and SF-PLGA(B) porous microspheres

Fig.5 DNA concentration quantitative analysis

Fig.6 Alkaline phosphatase activity assay

Fig.7 Optical microscopy images of PLGA(A) and SF-PLGA(B) after Alizarin red staining

Fig.8 SEM images(A,B) and EDS(C,D) of calcium nodules on PLAG(A, C) and SF-PLGA(B, D) porous microspheres

Fig.9 XRD patterns of PLGA(a, b) and SF-PLGA(c) porous microspheres a. Without cells; b,c. after osteogenic induction for 16 d.

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