Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (1): 47.doi: 10.7503/cjcu20180421
• Analytical Chemistry • Previous Articles Next Articles
LI Wenhong1, WANG Danyang2, CAO Jinjin2, WEI Yongju2,*()
Received:
2018-06-08
Online:
2019-01-10
Published:
2018-11-16
Contact:
WEI Yongju
E-mail:weiyju@126.com
Supported by:
TrendMD:
LI Wenhong,WANG Danyang,CAO Jinjin,WEI Yongju. Comparative Study of Absorption and Fluorescence Spectra of Glycitein and Glycitin†[J]. Chem. J. Chinese Universities, 2019, 40(1): 47.
pH | A | pKa1 | Average(pKa1) |
---|---|---|---|
6.85 | 0.2824 | 7.14 | 7.08±0.04 |
6.94 | 0.3206 | 7.10 | 7.08±0.04 |
7.00 | 0.3475 | 7.08 | 7.08±0.04 |
7.04 | 0.3856 | 7.01 | 7.08±0.04 |
Table 1 Determination of pKa1 of glycitein*
pH | A | pKa1 | Average(pKa1) |
---|---|---|---|
6.85 | 0.2824 | 7.14 | 7.08±0.04 |
6.94 | 0.3206 | 7.10 | 7.08±0.04 |
7.00 | 0.3475 | 7.08 | 7.08±0.04 |
7.04 | 0.3856 | 7.01 | 7.08±0.04 |
pH | A | pKa2 | Average(pKa2) |
---|---|---|---|
9.74 | 0.5511 | 9.95 | 9.96±0.01 |
9.86 | 0.5423 | 9.96 | 9.96±0.01 |
10.02 | 0.5311 | 9.97 | 9.96±0.01 |
10.21 | 0.5161 | 9.96 | 9.96±0.01 |
Table 2 Determination of pKa2 of glycitein*
pH | A | pKa2 | Average(pKa2) |
---|---|---|---|
9.74 | 0.5511 | 9.95 | 9.96±0.01 |
9.86 | 0.5423 | 9.96 | 9.96±0.01 |
10.02 | 0.5311 | 9.97 | 9.96±0.01 |
10.21 | 0.5161 | 9.96 | 9.96±0.01 |
Fig.3 Fluorescence spectra of glycitein aqueous solutions at different pH values(A) and relationship between fluorescence intensity and pH value(B) c: 1.56 μg/mL, volume fraction of MeOH: 10%, λex/λem: 347 nm/456 nm.
Fig.4 Absorption spectra of glycitin aqueous solutions at different pH values(A) and relationship between absorbance and pH value(B) c: 8.20 μg/mL, volume fraction of MeOH: 10%, λ=280 nm.
pH | A | pKa | Average(pKa) |
---|---|---|---|
9.63 | 0.3114 | 9.85 | 9.81±0.03 |
9.79 | 0.3342 | 9.87 | 9.81±0.03 |
9.90 | 0.3633 | 9.80 | 9.81±0.03 |
10.12 | 0.3946 | 9.81 | 9.81±0.03 |
Table 3 Determination of pKa of glycitin*
pH | A | pKa | Average(pKa) |
---|---|---|---|
9.63 | 0.3114 | 9.85 | 9.81±0.03 |
9.79 | 0.3342 | 9.87 | 9.81±0.03 |
9.90 | 0.3633 | 9.80 | 9.81±0.03 |
10.12 | 0.3946 | 9.81 | 9.81±0.03 |
Fig.5 Fluorescence spectra of glycitin alkaline solutions at different pH values(A) and influence of pH on fluorescence intensity(B) c: 1.68 μg/mL, volume fraction of MeOH: 10%, heat for 0.5 h at 100 ℃, λex/λem: 307 nm/415 nm.
Fig.7 Relationship between fluorescence intensity and heating temperaturec: 1.68 μg/mL, volume fraction of MeOH: 10%, heat for 0.5 h, pH=13.2, λex/λem: 307 nm/415 nm.
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