CJCU

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (8): 1768.

• Articles • Previous Articles     Next Articles

Prediction and Identification of Structural Proteins of Chinese Sacbrood Virus LN Isolate

MA Ming-Xiao1*, MA Chen2, CHENG Jian1, XIE Zhen-Sheng3, LI Ming1, FEI Dong-Liang1   

  1. 1. Department of Laboratory Animal Center Liaoning Medical University, Jinzhou 121001, China;
    2. Wengniuete Country Chinese Traditional Medicine and Mongolian Medicine in Chifeng Town, Wudan 024500, China;
    3. Proteomic Platform,   Institute of Biophysics,   Chinese Academy of Sciences, Beijing  100101, China
  • Received:2011-01-06 Revised:2011-03-18 Online:2011-08-10 Published:2011-07-19
  • Contact: MA Ming-Xiao E-mail:lnjzmmx@163.com
  • Supported by:

    国家自然科学基金(批准号: 30972200)和辽宁省教育厅项目(批准号: 2009A464)资助.

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Abstract: Abstract：Chinese sacbrood virus (CSBV) was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of CSBV is 8863 nucleotides in length and contains a single large open reading frame encoding a 319.614kDa polyprotein. The coding sequence is flanked by a 178-nucleotide 5' nontranslated leader sequence and a 142-nucleotide 3' nontranslated region, followed a poly(A) tail. Four major structural proteins, VP1,VP2, VP3 and VP4, were estimated, and mapped to the N-terminal section of the polyprotein of which the genes were predicted. Phylogenetic analysis using RdRp of selected picorna-like viruses shows that CSBV/SBV and Deformed Wing Virus (DWV) tend to group together, which possesses an RNA of similar size and gene order. The results predicted the probable size and gene order of CSBV/SBV. Structural proteins, VP1, VP3 and VP0, were identified after four proteins was analysed by mass spectrographic.

Key words: Chinese sacbrood virus(CSBV), RT-PCR, Structural protein; , Mass spectrographic analysis

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