CJCU

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (7): 1512.

• Preface • Previous Articles     Next Articles

Effect of  Tangshen Formula  on Phospholipids Metabolism of OLETF Rats

ZHU Chao1,2, HUANG Min1,2, LIANG Qiong-Lin2, WANG Yi-Ming2, HU Ping3, LI Ping4, ZHANG Hao-Jun4, LU Xiao-Guang4, LUO Guo-An1,2*   

  1. 1.  School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    2.  Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education,  Department of Chemistry, Tsinghua University, Beijing 100084, China; 
    3.  School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; 
    4.  Institute of Clinical Medical Research, China-Japan Friendship Hospital, Beijing 100029, China
  • Received:2010-08-16 Revised:2010-11-09 Online:2011-07-10 Published:2011-06-02
  • Contact: LUO Guo-An E-mail:luoga@mail.tsinghua.edu.cn
  • Supported by:

    国家自然科学基金(批准号:   90707045, 20805026, 90917003)和国家“九七三”计划项目(批准号:   2005CB523503, 2007CB714505)资助.

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Abstract: As one of the most common endocrine diseases, diabetic mellitus (DM) has a very close relationship with phospholipids metabolism. Tangshen Formula (TSF) is an often-used prescription with good efficacy for treating DM and diabetic nephropathy (DN). The aim of this study is to understand the effect of TSF on phospholipids metabolism of DM. In this paper, Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model of slowly progressive obese type 2 diabetes, were employed. Phospholipids profiling of rat plasma was established by using normal phase liquid chromatography-time of flight mass spectrometry (NPLC-TOF/MS). Partial least-squares discriminant analysis (PLS-DA) and quantification of 14 compounds were applied to evaluate the effect of TSF on phospholipids metabolism of OLETF rats and to discover potential biomarkers. The results showed plasma phospholipids metabolic disorders occurred in OLETF rats. The good effect of TSF on phospholipids metabolism was compared with the adverse impact of Monopril. TSF is able to significantly improve phospholipids metabolism of OLETF rats, whereas Monopril exacerbated disturbance of phospholipids metabolism. According to quantitative results, a total of 8 phospholipids compounds which have significant concentration variation between control group and model group were considered as potential biomarkers of type II diabetes.

Key words: Tangshen Formula, OLETF Rat, Phospholipids Metabolism, NPLC-TOF/MS, PLS-DA

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