CJCU

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (6): 1436.

• Articles • Previous Articles    

Preparation of Hexanediamine-contained PAMAM Dendrimers Supported on Chitosan Microspheres and Their Application in the Adsorption of Bilirubin

WU Li-Ping1,2, ZHANG Zheng-Pu1*, GUO Yi2, WANG Yong3, LU Wei3   

  1. 1. Institute of Polymer Chemistry, Key Laboratory of Functional Polymeric Materials, Ministry of Education, Nankai University, Tianjin 300071, China;
    2. Institute of Acupuncture, Key Laboratory of Medical Chemical Sensors of State Administration of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3. Haihe Hospital, Tianjin 300150, China
  • Received:2010-06-17 Revised:2010-08-16 Online:2011-06-10 Published:2011-05-10
  • Contact: ZHANG Zheng-Pu E-mail:zhangzp@nankai.edu.cn
  • Supported by:

    天津市应用基础及前沿技术研究计划(批准号: 08JCYBJC11300)资助.

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Abstract: Glutaraldehyde cross-linked chitosan microspheres (CS) were prepared by inverse phase suspension reaction. Low generations(Gn≤3)hexanediamine-contained PAMAM dendrimers were supported to chitosan microspheres. Bilirubin adsorption on the above microspheres was carried out in 0.05M phosphate buffer solution (pH=7.2-7.4) at 37℃. The results showed that the equilibrium adsorption percentage of CS-G2.0, CS-G3.0, CS-G1.0, CS-G0 and CS microspheres was 94.61%, 93.44%, 92.97%, 86.47%, 52.38%, respectively. The adsorption percentage of CS-G1.0-3.0 microspheres was over 70% at 0.5h and arrived to the equilibrium at about 1h.The adsorption capacity was up to 42.78mg/g. A ll the moderations (CS-G0-3.0) were much better than CS microspheres for bilirubin adsorption. The effects of temperature, pH, bilirubin initial concentration, ion strength and bovine serum albumin (BSA) on the adsorption properties for bilirubin were studied. It was shown that adsorption percentage increased at higher temperature. Adsorption properties were the best at near neutral solutions of pH≈7. PAMAM dendrimers supported in chitosan microspheres had satisfactory competitive abilities with BSA for bilirubin adsorption though adsorption percentage decreaed to a certain extent in the presence of BSA.

Key words: bilirubin adsorption, chitosan microsphere, hexanediamine(HDA)；methacrylate(MA)；polyamidoamine(PAMAM)

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