Abstract: DL5000 DNA Marker (100~5000 bp) fragments were separated through no-gel sieve capillary electrophoresis on a fused silica capillary column (31.2 cm×75 μm, the effective length 21.0 cm) using Poly (ethylene oxide) as sieve matrix. The influences of Poly (ethylene oxide) concentration, pH value of running buffer, separation voltage and ethidium bromide concentration on the separation efficiency of different lengths of DNA fragments were investigated. The optimum conditions for 100~5000 bp DNA fragments were established as 0.5% Poly (ethylene oxide), pH 8.0, 3.0 μg/mL ethidium bromide and voltage of 12 kV. Under these conditions, good resolution was obtained in separating multiplex polymerase chain reaction (PCR) magnified products from sorbitol dehydrogenase gene (SDH) and ethylene receptor gene (ETR1).

Key words: No-gel sieve capillary electrophoresis, Poly (ethylene oxide), DNA separation