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Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (2): 322.

• Articles • Previous Articles     Next Articles

Preparation and Application in Capturing Bacteria of Submicron Immunomagnetic Beads

NIU Mu1, DU Mei-Hong2, DENG Yi1,2, GAO Ming-Yuan1*   

  1. 1. Institue of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    2. Beijing Center for Physical and Chemical Analysis, Beijing 100089, China
  • Received:2010-11-01 Revised:2010-11-18 Online:2010-02-10 Published:2011-02-23
  • Contact: GAO Ming-Yuan E-mail:gaomy@iccas.ac.cn
  • Supported by:

    国家卫生部重大专项(批准号: 2008ZX10001-014)和中国科学院知识创新工程(批准号: KJCX2-YW-M15)资助.

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Abstract: Based on the submicron monodispersed magnetic beads, the immunomagnetic beads covered with anti-Salmonella antibody were obtained. The adsorption behavior of IgG on the surface of magnetic beads was investigated through surface electric potential, fluorescence and ELISA method. In the immunoassay experiment, by adjusting the initial concentration of IgG, the relativity between surface concentration of IgG on magnetic beads and magnetic capture efficiency was also investigated. Finally, comparison with commercial Dynabeads, our submicron immunomagnetic beads represents higher capture efficiency for Salmonella.

Key words: submicron, immunomagnetic beads, Salmonella, magnetic capture efficiency

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