CJCU

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (2): 258.

• Articles • Previous Articles     Next Articles

Impact of Orchiectomy on the Adrenal Metabolism of Pregnenolone, \%17α\%\|Hydroxypregnenolone and Dehydroepiandrosterone in Rats

HUA Hai-Yin1, CHEN Jun1, FAN Xue-Mei2, HU Ming-Qiu3, LIANG Qiong-Lin1*, NA Yan-Qun3, WANG Yi-Ming1, LUO Guo-An1*   

  1. 1. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua Unisversity, Beijing 100084, China;
    2. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    3. Institute of Urology, First Hospital of Peking University, Beijing 100034, China
  • Received:2010-05-31 Revised:2010-06-29 Online:2010-02-10 Published:2011-02-23
  • Contact: LUO Guo-An;LIANG Qiong-Lin E-mail:luoga@mail.tsinghua.edu.cn;liangql@tsinghua.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 30772169, 20805026, 90917003)资助.

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Abstract: Androgen deprivation therapy, the main method for clinical prostate cancer, had been proved to be able to promote the expression of CYP17a1 gene in adrenal. As CYP17a1 was the crucial enzyme for the production of 17α-hydroxypregnenolone and dehydroepiandrosterone, we established a GC-MS method to illustrate the change of pregnenolone, 17α-hydroxypregnenolone, and dehydroepiandrosterone after orchiectomy in emasculated rat adrenal. 4 weeks after orchiectomy, there was a significant increase of 17α-hydroxypregnenolone and dehydroepiandrosterone accompanied with the increase of CYP17a1 gene expression 2 weeks after orchiectomy in adrenal.

Key words: orchiectomy, adrenal； gas chromatography with mass spectrometry； 17α-Hydroxypregnenolone； dehydroepiandrosterone

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