Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (7): 1337.

• Articles • Previous Articles     Next Articles

Conformation Research of Shorted Glucagon-like Peptide-1

NIE Xin-Hua1, ZHANG Tao1, LI Yuan2*, ZHENG Xue-Fang1*, CAO Hong-Yu1, TANG Qian1   

  1. 1. College of Bioengineering, Dalian University, Dalian 116622, China;
    2. Dalian DN Bio-engineering Co., Ltd., Dalian 116600, China
  • Received:2009-12-08 Online:2010-07-10 Published:2010-07-10
  • Contact: LI Yuan. E-mail: dalianliyuan@yaoo.com.cn;ZHENG Xue-Fang. E-mail: dlxfzheng@163.com
  • Supported by:

    国家“十一五”计划科技重大专项“重大医药创制”项目(批准号: 2009zx09102-53)、辽宁省高校创新团队项目(批准号: 2006T002, 2008T005, 2009T003)和大连市科技计划项目(批准号: 2008E11SF170)资助.

Abstract:

Shorted glucagon-like peptide-1(sGLP-1) not only stimulate proliferation of pancreatic beta cells and decrease blood glucose, but also has a longer half-life. This make sGLP-1 has become a potential new drug in the future. In order to elucidate the function of sGLP-1, this study investigates the conformation of sGLP-1 in different conditions by circular dichroism spectrum and fluorescence spectrum. The conformations of sGLP-1 in phosphate buffer are mostly unordered structure and very low helix, and are mostly α-helix in phosphate buffer containing SDS or TFE. Temperature and pH values of solutions have great influences on the conformation of sGLP-1. sGLP-1 preserve the critical residues which are responsible for binding and activating receptor, Induced by receptor, sGLP-1 can form the correct conformation which can bind and activate receptor in vivo, then realize its function. The results show that appropriately truncate C-terminal segment of GLP-1, may obtain long-term and high efficiency GLP-1 analogues.

Key words: Shorted glucagon-like peptide-1; Circular dichroism spectrum; Fluorescence spectrum; Conformation

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