Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (7): 1327.

• Articles • Previous Articles     Next Articles

Quantification of Somatostatin with a Surface Plasmon Resonance-based Biosensor

GAO Jin-Hang1, LIU Rui1, TONG Huan2, LI Xian1, QIANG Ou1, TANG Cheng-Wei1,2*   

  1. 1. State Key Laboratory of Biotherapy,
    2. Departement of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2009-12-14 Online:2010-07-10 Published:2010-07-10
  • Contact: TANG Cheng-Wei. E-mail: cwtang@medmail.com.cn
  • Supported by:

    国家自然科学基金重点项目(批准号: 30330270)资助.

Abstract:

A quick technique of quantifying somatostatin(SST) with surface plasmon resonance(SPR) on the principle of antigen-antibody-antigen assay was established. The optimal coupling of CM5 sensor chip for SST was indicated as 1231.4 response unit(RU) at pH=5.0, and the SST concentration of 75 μg/mL. A negative linear relationship between SST content(20—2000 pg) and RU was obtained using the high molecular mass antibody for SST at 117.7 nmol/L, R=-0.824, p<0.05, coefficient of variation 1.3%. This method can be used to detect SST content at a range from 20 to 2000 pg with good repetition and real-time, which would be useful for clinical diagnosis and intensive care of critically ill patients.

Key words: Quantification of somatostatin; Surface plasmon resonance; Antigen-antibody-antigen binding assay

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