CJCU

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (6): 1280.

• Articles • Previous Articles    

Synthesis, Self-assembly and Gene Transfection Hyperbranched Polyethylenimine-poly(β-benzyl-L-aspartate) Copolymer

JIAO Yong-Hua, ZHOU Jun, LI Yan, ZHANG Kai, FU Yu*   

  1. College of Sciences, Northeastern University, Shenyang 110004, China
  • Received:2009-08-24 Online:2010-06-10 Published:2010-06-10
  • Contact: FU Yu. E-mail: fuyu@mail.neu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20704003)和超分子结构与材料国家重点实验室开放课题(批准号: SKLSSM200906)资助.

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Abstract:

A novel amphiphilic and biodegradable copolymer of hyperbranched polyethylenimine-poly(β-benzyl-L-aspartate)(PEI-PBLA) was synthesized by ring opening polymerization of the N-carboxyanhydride of β-benzyl-L-aspartate(BLA-NCA) with hyperbranched polyethylenimine(PEI) as initiator and characterized by 1H NMR. PEI-PBLA copolymers could form micelles in both organic solvent and water. The micelle formation of PEI-PBLA in CH2Cl2 was displayed by extraction of methyl orange indicator from aqueous solutions. As for PEI-PBLA micelle in water, the critical micelle concentration(cmc) was determined by fluorescence probe method and corresponding morphology was observed by TEM. Meanwhile, the complexation of PEI-PBLA with DNA was explored by gel retardation. The studies imply that PEI-PBLA has great potential in many applications, such as drug delivery, gene transfection and so on.

Key words: Hyperbranched polyethylenimine(PEI); Poly(β-benzyl-L-aspartate); Polymer micelle; Gene vector

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