Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (6): 1148.

• Articles • Previous Articles     Next Articles

Synthesis of pH Probe Based on Styrylcyanine Dyes and Fluorescence Image in Live Cell

WANG Bing-Shuai, WANG Li, WU Tong, YANG Zhi-Gang, PENG Xiao-Jun*   

  1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
  • Received:2009-09-25 Online:2010-06-10 Published:2010-06-10
  • Contact: PENG Xiao-Jun. E-mail: pengxj@dlut.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20406004, 20572012, 20536010)资助.

Abstract:

Fluorescent pH indicators have been widely applied in recent years to monitor changes in intracellular pH. Though some pH probes were synthesized and applied to sense the acidic organelles in live cells. However, the syntheses of these probes were very complicated. So, one pH fluorescence probe based on amino-styrylcyanine was synthesized with good yield and its purification was easy. Its abilities as pH probe were tested in ethanol-water solution. The results showed the absorption intensity of the base form(short-wavelength) decreased with the decreasing of pH, at the same time, the absorption intensity of the protonated forms(long-wavelength) increased. The color of the solution changed from yellow to pink. The base form had no fluorescence, but the fluorescence intensity of the protonated form increased remarkably. The pKa of the probe was about 4.97. Live cell fluorescence images indicated the probe possesses good cell membrane permeation and can associate with lower pH yield inside the cytosol which can be applied to sense acidic organelles in live cells.

Key words: Styrylcyanine dye; pH probe; Live cell fluorescence image; Acidic organelle

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