Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (5): 964.

• Articles • Previous Articles     Next Articles

Modified Citrus Pectin: Preparation, Characterization and Anti-cancer Activities

ZHANG Wen-Bo1*, LIU Chang-Zhong2, GAO Lin1   

  1. 1. School of Pharmaceutical and Technology, Tianjin University, Tianjin 300072, China;
    2. School of Animal Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China
  • Received:2009-07-02 Online:2010-05-10 Published:2010-05-10
  • Contact: ZHANG Wen-Bo. E-mail: zhwenbo@gmail.com

Abstract:

Agarose gel electrophoresis, specific rotatory, HPSEC-RID, IR, 1H NMR, 13C NMR, periodate oxidation, GC-MS and methylation analysis were applied to investigate the homogeneity, chemical properties and structural features of modified citrus pectin(MCP). Results demonstrated that MCP was a homogeneous polysaccharide. Its molecular weight ranged from 21000 to 66000; the content of galacturonic acid was 81.0% and the degree of esterification was 2.13%. MCP contains Gal, Xyl, Rha and Ara; their molar ratio was 1.0∶1.5∶1.4∶1.3. Methylation analysis results confirmed that MCP had neutral sugar residues composed of Xyl, Ara and terminal Gal. Heptoma H22 was significantly inhibited by MCP and the inhibitor rate was 47.8%(n=10, p<0.01) at high-dose level(4 mg/kg). MCP also demonstrated anti-cervical carcinoma activity in U14 mice model. No anti-cancer activity was observed in Sarcoma 180 group.

Key words: Modified citrus pectin; Polysaccharide; Anti-cancer activity

TrendMD: