Construction Mechanism of Nanometer Pyronine G Core-Ferritin Revealed with Circular Dichroism and Fluorometry

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (5): 896.

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Construction Mechanism of Nanometer Pyronine G Core-Ferritin Revealed with Circular Dichroism and Fluorometry

CHEN Ying-Ying¹, HUANG Lin^1,3, JI Xue-Tao¹, LIN Qing¹, CHEN Ping¹, KE Cai-Huan², HUANG He-Qing^1,2,3*

1. Department of Biochemistry and Biotechnology, School of Life Sciences,
2. State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science,
3. Key Laboratory of Chemical Biology of Fujian Province, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received:2009-07-22 Online:2010-05-10 Published:2010-05-10
Contact: HUANG He-Qing. E-mail: hqhuang@xmu.edu.cn
Supported by:
国家自然科学基金(批准号: 30870515)和国家“九七三”计划项目(批准号: 2010CB12640)资助.

Abstract

Abstract:

Liver ferritin of Sphyrna zygaena(SZLF) with electrophoresis purity was prepared in bath to study its structure and function in batch. Under the condition of pH=2.0—10.0, circular dichroism and fluorometry were used to study the primary structural characteristics and change trend of secondary structure conversion both SZLF and apoSZLF, respectively, and revealed the stability, the interaction intensity, and the unfold behavior among subunits of SZLF shell. Using the approach of acidic and basic neutralization, the subunits of protein shell in SZLF can be disassociated, and these subunits disassociated can be also recombined into another new complete ferritin again, which sets up a reasonable technology for constructing a nanometer pyronine G core-ferritin(NPGCF). Both UV-Vis spectrophotometry and fluorometry were used to study the construction pathways of NPGCF, giving quantificationally twelve pyronine G molecules that were directly trapped into its protein shell for constructing NPGCF by each apoSZLF molecule. In addition, these techniques not only analyze both the pathway and the rate for trapping and releasing pyronine G, but also construct another new drug carrier of nanometer cisplatin core with high carrying capacity-ferritin.

Key words: Ferritin; Structure conversion; Nanometer pyronine G core-ferritin; Spectrum analysis

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CHEN Ying-Ying, HUANG Lin, JI Xue-Tao, LIN Qing, CHEN Ping, KE Cai-Huan, .... Construction Mechanism of Nanometer Pyronine G Core-Ferritin Revealed with Circular Dichroism and Fluorometry[J]. Chem. J. Chinese Universities, 2010, 31(5): 896.

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Construction Mechanism of Nanometer Pyronine G Core-Ferritin Revealed with Circular Dichroism and Fluorometry

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