Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 632.

• Letters • Previous Articles     Next Articles

Organic Two-photon Fluorescence Nanoparticles for Cell Stains

XU Hong1*, YANG Shu-Min2, CHEN Geng1, TIAN Lei-Lei2, HE Feng2, WANG Guang-Yi1*, MA Yu-Guang2   

  1. 1. The First Hospital of Jilin University, Changchun 130021, China;
    2. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
  • Received:2009-10-04 Online:2010-04-10 Published:2010-04-10
  • Contact: XU Hong. E-mail: chxuhong@163.com; WANG Guang-Yi. E-mail: wgymd@sina.com
  • Supported by:

    国家自然科学基金(批准号: 20834006)资助.

Abstract:

Two-photon absorption materials offer great promise as the new generation of fluorescent probes to image and study biological processes. We present water-dispersed two-photon functional organic nanoparticles which have been fabricated via reprecipitation method based on two-photon absorbing molecule 2,5,2′,5′-tetra(4′-N,N-diphenylaminostyryl)biphenyl(DPA-TSB), aiming to increase active dye loading density and enhance sensitive bioimaging in the microscopic level. In vitro confocal microscopy has demonstrated that these nanoparticles are avidly internalized into cells with bright fluorescence. The good cell membrane permeability of two-photon functional DPA-TSB nanoparticles make them use as bioimaging staining agent for the deeper tissue.

Key words: Two-photon fluorescence; Organic nanoparticle; Cell stain

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