Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 629.

• Letters • Previous Articles     Next Articles

New Method for Dynamic Visualization of Poliomyelitis Virus Infection Process

WANG Ping-Cui, XIE Hai-Yan*, ZHOU Xi-Ye, WANG Yang   

  1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China
  • Received:2009-12-28 Online:2010-04-10 Published:2010-04-10
  • Contact: XIE Hai-Yan. E-mail: hyanxie@bit.edu.cn
  • Supported by:

    国家“九七三”计划项目(批准号: 2006CB933100)、国家自然科学基金(批准号: 20975013)、教育部新世纪优秀人才支持计划项目(批准号: NCET-08-0046)、卫生部科技重大专项(批准号: 2009ZX10004-107)和北京理工大学优秀青年教师资助计划择优二次资助基金(批准号: 2007YS0603)资助.

Abstract:

The propagative polioviruses were modified with biotin firstly and then labeled with quantum dots through biotin-streptavidin system. On the basis of the strong fluorescence intensity and high photostability of quantum dots, a new method for long-term tracking of single-viruses was established with a live-cell confocal microscope imaging system. The results of the real-time dynamic imaging of QDs-labeled poliovirus showed that the entry process of QDs-labeled poliovirus was rapid and actin-dependent. The method is simple and versatile. It can be applied to the study of infectious mechanism of all kinds of enveloped viruses.

Key words: Quantum dots; Virus; Infection; Real time; Dynamic

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