Multi-walled Carbon Nanotubes Induces Nuclear Factor-κB Activation in A549 Cells

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (3): 497.

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Multi-walled Carbon Nanotubes Induces Nuclear Factor-κB Activation in A549 Cells

YE She-Fang^1*, WEN Wen¹, WANG Yi-Fang¹, LIN Cui-Lin¹, WU Yi-Hui¹, ZHANG Qi-Qing^1,2*

1. Research Center of Biomedical Engineering&Department of Biomaterial, Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering Technology of Xiamen City, College of Materials, Xiamen University, Xiamen 361005, China
2. Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peiking Union Medical College, Key Laboratory of Biomedical Materials of Tianjin, Tianjin, 391002, China

Received:2009-06-01 Online:2010-03-10 Published:2010-03-10
Contact: YE She-Fang. E-mail: yeshefang@xmu.edu.cn; ZHANG Qi-Qing. E-mail: zhangqiq@xmu.edu.cn
Supported by:
国家重大科学研究计划项目(批准号: 2006CB933300)、国家自然科学基金(批准号: 30901175)和福建省青年人才项目(批准号: 2008F3097)资助.

Abstract

Abstract:

The present study was undertaken to examine the effects of multi-walled carbon nanotubes(MWCNTs) on nuclear factor(NF)-κB activation in human A549 lung epithelial cells. Cultured A549 cells were stimulated with various concentrations of MWCNTs in the presence or absence of antioxidants. The intracellular generation of reactive oxygen species(ROS) was detected by means of flow cytometry analysis with a redox-sensitive fluorescent probe 2′,7′-dichlorfluorescein-diacetate. NF-κB activity was measured by electrophoretic mobility shift assay(EMSA). The protein levels of p65 and IκBα were assessed by Western blot analysis. Translocation of p65 into the nucleus was examined by immunofluorescence confocal microscopy. The results showed increased ROS and NF-κB activity in response to treatment of A549 cells with MWCNTs. MWCNTs stimulated nuclear translocation of the p65 subunit of NF-κB, IκBα phosphorylation in A549 cells. Treatment of A549 cells with antioxidants prior to adding MWCNTs resulted in a significant reduction in ROS generation, NF-κB activity, p65 translocation and IκBα phosphorylation in A549 cells. These results indicate that MWCNTs are able to induce NF-κB activation in A549 cells mediated by oxidative stress.

Key words: Multi-walled carbon nanotube; Oxidative stress; Nuclear factor-κB

TrendMD:

YE She-Fang*, WEN Wen, WANG Yi-Fang, LIN Cui-Lin, WU Yi-Hui, ZHANG Qi-Qing. Multi-walled Carbon Nanotubes Induces Nuclear Factor-κB Activation in A549 Cells[J]. Chem. J. Chinese Universities, 2010, 31(3): 497.

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Multi-walled Carbon Nanotubes Induces Nuclear Factor-κB Activation in A549 Cells

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