Studies on Gallbladder Tissues, Tissue Plasma Membranes and Cell Lines by FTIR

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (3): 484.

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Studies on Gallbladder Tissues, Tissue Plasma Membranes and Cell Lines by FTIR

WANG Jian-Sheng^1*, ZHANG Jia¹, WU Wen-An¹, DUAN Xiao-Yi¹, WANG Si-Cen³, ZHANG Ming-Xin¹, ZHOU Su-Na¹, MO Fei¹, XU Yi-Zhuang², SHI Jing-Sen¹, WU Jin-Guang²

1. Department of Surgery of Oncology, First Hospital of Xi′an Jiaotong University, Xi′an 710061, China;
2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
3. School of Medicine, Xi′an Jiaotong University, Xi′an 710061, China

Received:2009-05-15 Online:2010-03-10 Published:2010-03-10
Contact: WANG Jian-Sheng. E-mail: wangjsh@mail.xjtu.edu.cn
Supported by:
国家自然科学基金(批准号: 30500119)和国家“九七三”计划前期专项基金(批准号: 2002CCA01900)资助.

Abstract

Abstract:

12 Cancer tissue samples, 14 inflammatory samples and 10 normal samples of gallbladder and their corresponding tissue membranes and gallbladder cancer cell lines were observed via FTIR. Compared to normal gallbladder tissue, the intensities of bands, related to lipids, at 2955, 2920, 2870, 2850 and 1740 cm^-1, decreased for gallbladder cancer tissue, the intensity of bands, related to protein, at 1550 cm^-1 decreased for gallbladder cancer tissue; the intensities of lipids bands and protein bands increased for gallbladder cancer cell line obviously; the intensities of lipids related bands also increased for gallbladder cancer tissue membrane; the intensity of band related to nucleic acid for gallbladder cancer tissue is similar to that for gallbladder cancer cell line and the band disappeared for gallbladder cancer tissue plasma membrane; the ratio of I₁₄₆₀/I₁₄₀₀ changed uniformity for tissue, cell line and tissue plasma membrane, which is smaller for cancer ones and bigger for normal ones. These results indicate that compared with those of gallbladder cancer tissue, the FTIR spectra changed a lot for gallbladder cancer cell lines and gallbladder cancer tissue plasma membrane. Protein and lipids relatively decreased in gallbladder cancer tissues but increased in gallbladder cancer cell line and gallbladder cancer tissue plasma membrane. The paper expounds FTIR variation for carcinogenesis from tissue, cell line and tissue plasma membrane. The ratio of I₁₄₆₀/I₁₄₀₀ changed uniformity for tissue, cell line and tissue plasma membrane, more studies may get a fine clinical diagnosis index.

Key words: Gallbladder; Gallbladder cancer; Plasma membrane; Gallbladder cancer cell line; FTIR

TrendMD:

WANG Jian-Sheng*, ZHANG Jia, WU Wen-An, DUAN Xiao-Yi, WANG Si-Cen, .... Studies on Gallbladder Tissues, Tissue Plasma Membranes and Cell Lines by FTIR[J]. Chem. J. Chinese Universities, 2010, 31(3): 484.

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Studies on Gallbladder Tissues, Tissue Plasma Membranes and Cell Lines by FTIR

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