Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (3): 442.

• Articles • Previous Articles     Next Articles

Effect of Zn-Chlorophyll Prepared from Silkworm Faeces on DNA Photocleavage

SI Li-Ping1, LIU Jing-Jing1, SHI Lei1, WANG Xiang-Li1, ZHANG Lei1, LIU Hai-Yang1*, JI Liang-Nian2   

  1. 1. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China;
    2. School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China
  • Received:2009-07-26 Online:2010-03-10 Published:2010-03-10
  • Contact: LIU Hai-Yang. E-mail: chhyliu@scut.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20771039, 20871122, 20971046)资助.

Abstract:

Zinc chlorophyll(Zn-CHL) complex was prepared using chlorophyll extacted from silkworm faeces. Relative viscosity, UV-Visible and CD spectra were used to study the interaction between Zn-CHL and ct-DNA. Agarose gel electrophoresis was also carried out to investigate the photocleavage ability of Zn-CHL to pBR 322 DNA. The results suggested that the binding mode of Zn-CHL and ct-DNA was outside binding, the binding constant was 2.32×105 L/mol. Agarose gel electrophoresis picture showed that Zn-CHL could induce DNA cleavage upon irradiation.

Key words: Chlorophyll; Silkworm faeces; DNA; Photocleavage

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