Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (2): 293.

• Articles • Previous Articles     Next Articles

Determination of AR Gene Expression in Diabetic Nephropathy

HE Yong-Xin1, LI Xue2, FAN Xue-Mei3, WANG Wan3, WANG Yi-Ming1, LUO Guo-An1,3*   

  1. 1. Department of Chemistry,
    2. Department of Medical Science, Tsinghua University, Beijing 100084, China;
    3. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • Received:2009-01-23 Online:2010-02-10 Published:2010-02-10
  • Contact: LUO Guo-An. E-mail: luoga@mail.tsinghua.edu.cn
  • Supported by:

    国家“九七三”计划项目(批准号: 2005CB523503)资助.

Abstract:

Diabetic nephropathy(DN) is a common chronic diabetic microvascular complication. DN is closely related to polyol pathway of glycometabolism, and aldose reductase(AR) is a rate-limiting enzyme in polyol pathway. In order to study the AR expression level in patients with different stages of DN, AR gene expression was detected in 50 cases of normal subjects and 80 diabetic nephropathy patients with different stages. The results show that AR gene is expected to be a biomarker for early diagnosis of DN, as well as the potential drug target in DN treatment. According to AR gene expression, the diagnosis of diabetic nephropathy in Chinese medicine is verified on the molecular biology level.

Key words: Diabetic nephropathy; Aldose reductase; Taqman real-time PCR

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