Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (2): 274.

• Articles • Previous Articles     Next Articles

Simple Method of Protein Detection Based on Double-stranded Fluorescent Aptamer Probe

GUO Qiu-Ping, YANG Xiao-Hai, WANG Ke-Min*, MENG Xiang-Xian, LI Jun, ZHONG Zhi-Hong   

  1. State Key of Laboratory of Chemo/biosensing and Chemomertrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Institute of Biological Technology, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2009-06-08 Online:2010-02-10 Published:2010-02-10
  • Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn
  • Supported by:

    国家“九七三”计划项目(批准号: 2002CB513110)、科技部国际合作重点项目(批准号: 2003DF000039)、国家自然科学基金(批准号: 90606003, 20805012)和湖南省杰出青年科学基金(批准号: 08JJ1002)资助.

Abstract:

A simple method of protein detection was developed using a double-stranded fluorescent aptamer probe. This double-stranded aptamer probe consisted of an aptamer probe labeled with a fluorophore and a short complementary oligonucleotide labeled with a quencher. In the presence of protein, the double-stranded aptamer probe dissociated and F-aptamer/protein complex was formed, leading to fluorescence restoring. Then protein detection was carried out by monitoring fluorescence enhancement, with a liner range of 6—100 nmol/L and detection limit of 6 nmol/L. This design strategy is easy to generalize for any aptamer without prior knowledge of its secondary or tertiary structure, and would be used as a simple and general tool for protein detection.

Key words: Aptamer; Protein detection; Thrombin

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